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DNA purification using a novel γ-Fe2O3/PEDOT hybrid nanocomposite.
da Silva, Romário J; Pedro, Graciela C; Gorza, Filipe D S; Maciel, Bruna G; Ratkovski, Gabriela P; Mojica-Sánchez, Lizeth Carolina; Medina-Llamas, Juan C; Chávez-Guajardo, Alicia E; de Melo, Celso P.
Afiliación
  • da Silva RJ; Departamento de Física, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
  • Pedro GC; Programa de Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
  • Gorza FDS; Fundação Universidade Federal de Rondônia - UNIR, 76801-974, Porto Velho, RO, Brazil.
  • Maciel BG; Programa de Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
  • Ratkovski GP; Departamento de Física, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
  • Mojica-Sánchez LC; Departamento de Física, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
  • Medina-Llamas JC; Centro de Estudios Científicos y Tecnológicos No.18, Instituto Politécnico Nacional, 98160, Zacatecas, Zac, Mexico.
  • Chávez-Guajardo AE; Unidad Académica de Ciencias de la Tierra, Universidad Autónoma de Zacatecas, 98058, Zacatecas, Zac, Mexico.
  • de Melo CP; Programa de Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil; Departamento de Física, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil. Electronic address: celso.melo@ufpe.br.
Anal Chim Acta ; 1178: 338762, 2021 Sep 15.
Article en En | MEDLINE | ID: mdl-34482873
We report the synthesis and characterization of a new hybrid magnetic composite formed by the enveloping of magnetic iron oxide nanoparticles (γ-NP) with chains of the conductive polymer PEDOT, and its use for the efficient separation of DNA molecules from complex biological samples, allowing the high yield separation of a pure and high-quality DNA fraction. The successful formation of the γ-NP/PEDOT composite was confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy, UV visible spectroscopy (UV-Vis), and magnetic hysteresis loop measurements. The nanocomposites showed an excellent capacity of DNA adsorption (Qe âˆ¼ 248 mg/g) in a model system consisting of salmon sperm DNA. When the γ-NP/PEDOT was used in protocols to extract the DNA from complex samples, the corresponding yield was in the range of 6.4 µg (blood) and 7.3 µg (bacteria), as evaluated quality by UV-Vis, PCR analysis, and electrophoresis assays. We also established that the captured DNA does not need to be detached from the nanocomposite for use as seeding material in PCR amplification experiments. These results and the simplicity of the protocols indicate that the γ-NP/PEDOT composite is a promising DNA absorbent, being competitive with the commercially available magnetic purification kits.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanocompuestos Idioma: En Revista: Anal Chim Acta Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanocompuestos Idioma: En Revista: Anal Chim Acta Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos