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Performance of xylose-fermenting yeasts in oat and soybean hulls hydrolysate and improvement of ethanol production using immobilized cell systems.
Cortivo, Paulo Roberto Dall; Aydos, Luiza Fichtner; Hickert, Lilian Raquel; Rosa, Carlos Augusto; Hector, Ronald E; Mertens, Jeffrey A; Ayub, Marco Antônio Záchia.
Afiliación
  • Cortivo PRD; Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Aydos LF; Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Hickert LR; State University of Rio Grande do Sul, Av. Bento Gonçalves, 8855, Porto Alegre, RS, 91540-000, Brazil.
  • Rosa CA; Department of Microbiology, ICB, C.P. 486, State University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
  • Hector RE; Bioenergy Research Unit, USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL, 61604, USA.
  • Mertens JA; Bioenergy Research Unit, USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL, 61604, USA.
  • Ayub MAZ; Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br.
Biotechnol Lett ; 43(10): 2011-2026, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34480641
We investigated the fermentation of a mixture of oat and soybean hulls (1:1) subjected to acid (AH) or enzymatic (EH) hydrolyses, with both showing high osmotic pressures (> 1200 Osm kg-1) for the production of ethanol. Yeasts of genera Spathaspora, Scheffersomyces, Sugiymaella, and Candida, most of them biodiverse Brazilian isolates and previously untested in bioprocesses, were cultivated in these hydrolysates. Spathaspora passalidarum UFMG-CM-469 showed the best ethanol production kinetics in suspended cells cultures in acid hydrolysate, under microaerobic and anaerobic conditions. This strain was immobilized in LentiKats® (polyvinyl alcohol) and cultured in AH and EH. Supplementation of hydrolysates with crude yeast extract and peptone was also performed. The highest ethanol production was obtained using hydrolysates supplemented with crude yeast extract (AH-CYE and EH-CYE) showing yields of 0.40 and 0.44 g g-1, and productivities of 0.39 and 0.29 g (L h)-1, respectively. The reuse of the immobilized cells was tested in sequential fermentations of AH-CYE, EH-CYE, and a mixture of acid and enzymatic hydrolysates (AEH-CYE) operated under batch fluidized bed, with ethanol yields ranging from 0.31 to 0.40 g g-1 and productivities from 0.14 to 0.23 g (L h)-1. These results warrant further research using Spathaspora yeasts for second-generation ethanol production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Xilosa / Células Inmovilizadas / Etanol / Saccharomycetales Idioma: En Revista: Biotechnol Lett Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Xilosa / Células Inmovilizadas / Etanol / Saccharomycetales Idioma: En Revista: Biotechnol Lett Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos