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Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice.
Do Pham, Duy Dinh; Jencová, Vera; Kanuchová, Miriam; Bayram, Jan; Grossová, Ivana; Suca, Hubert; Urban, Lukás; Havlícková, Kristýna; Novotný, Vít; Mikes, Petr; Mojr, Viktor; Asatiani, Nikifor; Kostáková, Eva Kuzelová; Maixnerová, Martina; Vlková, Alena; Vítovská, Dragana; Sanderová, Hana; Nemec, Alexandr; Krásný, Libor; Zajícek, Robert; Lukás, David; Rejman, Dominik; Gál, Peter.
Afiliación
  • Do Pham DD; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nám. 2, 166 10, Prague 6, Czech Republic.
  • Jencová V; Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17, Liberec 1, Czech Republic.
  • Kanuchová M; Laboratory of Cell Interactions, Center of Clinical and Preclinical Research MediPark, Pavol Jozef Safárik University, Trieda SNP 1, 040 11, Kosice, Slovak Republic.
  • Bayram J; Prague Burn Centre, Third Faculty of Medicine, University Hospital Královské Vinohrady, Srobárova 50, 100 34, Prague 10, Czech Republic.
  • Grossová I; Prague Burn Centre, Third Faculty of Medicine, University Hospital Královské Vinohrady, Srobárova 50, 100 34, Prague 10, Czech Republic.
  • Suca H; Prague Burn Centre, Third Faculty of Medicine, University Hospital Královské Vinohrady, Srobárova 50, 100 34, Prague 10, Czech Republic.
  • Urban L; Laboratory of Cell Interactions, Center of Clinical and Preclinical Research MediPark, Pavol Jozef Safárik University, Trieda SNP 1, 040 11, Kosice, Slovak Republic.
  • Havlícková K; Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Ondavská 8, 040 11, Kosice, Slovak Republic.
  • Novotný V; Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17, Liberec 1, Czech Republic.
  • Mikes P; The Institute for Nanomaterials, Advanced Technologies and Innovation, Bendlova 1409/7, 460 01, Liberec 1, Czech Republic.
  • Mojr V; Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17, Liberec 1, Czech Republic.
  • Asatiani N; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nám. 2, 166 10, Prague 6, Czech Republic.
  • Kostáková EK; Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17, Liberec 1, Czech Republic.
  • Maixnerová M; Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17, Liberec 1, Czech Republic.
  • Vlková A; Laboratory of Bacterial Genetics, Centre for Epidemiology and Microbiology, National Institute of Public Health, Srobárova 49/48, 100 00, Prague 10, Czech Republic.
  • Vítovská D; Unit for Biomedicine and Welfare of Laboratory Animals, National Institute of Public Health, Srobárova 49/48, 100 00, Prague 10, Czech Republic.
  • Sanderová H; Laboratory of Microbial Genetics and Gene Expression, Institute of Microbiology, Czech Academy of Sciences V.V.I., Vídenská 1083, 142 20, Prague 4, Czech Republic.
  • Nemec A; Laboratory of Microbial Genetics and Gene Expression, Institute of Microbiology, Czech Academy of Sciences V.V.I., Vídenská 1083, 142 20, Prague 4, Czech Republic.
  • Krásný L; Laboratory of Bacterial Genetics, Centre for Epidemiology and Microbiology, National Institute of Public Health, Srobárova 49/48, 100 00, Prague 10, Czech Republic.
  • Zajícek R; Department of Medical Microbiology, Second Faculty of Medicine, Charles University, V Úvalu 84, 150 06, Prague 5, Czech Republic.
  • Lukás D; Laboratory of Microbial Genetics and Gene Expression, Institute of Microbiology, Czech Academy of Sciences V.V.I., Vídenská 1083, 142 20, Prague 4, Czech Republic.
  • Rejman D; Prague Burn Centre, Third Faculty of Medicine, University Hospital Královské Vinohrady, Srobárova 50, 100 34, Prague 10, Czech Republic.
  • Gál P; Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17, Liberec 1, Czech Republic.
Sci Rep ; 11(1): 17688, 2021 09 03.
Article en En | MEDLINE | ID: mdl-34480072
Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of wound dressings based on the polycaprolactone nanofiber scaffold (NANO) releasing second generation lipophosphonoxin (LPPO) as antibacterial agent. Firstly, we demonstrated in vitro that LPPO released from NANO exerted antibacterial activity while not impairing proliferation/differentiation of fibroblasts and keratinocytes. Secondly, using a mouse model we showed that NANO loaded with LPPO significantly reduced the Staphylococcus aureus counts in infected wounds as evaluated 7 days post-surgery. Furthermore, the rate of degradation and subsequent LPPO release in infected wounds was also facilitated by lytic enzymes secreted by inoculated bacteria. Finally, LPPO displayed negligible to no systemic absorption. In conclusion, the composite antibacterial NANO-LPPO-based dressing reduces the bacterial load and promotes skin repair, with the potential to treat wounds in clinical settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus / Vendajes / Cicatrización de Heridas / Infección de Heridas / Nanofibras / Antibacterianos Límite: Animals Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus / Vendajes / Cicatrización de Heridas / Infección de Heridas / Nanofibras / Antibacterianos Límite: Animals Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido