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Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect.
Moradi, Aboubakr; Austerlitz, Tina; Dahlin, Paul; Robert, Christelle Am; Maurer, Corina; Steinauer, Katja; van Doan, Cong; Himmighofen, Paul Anton; Wieczorek, Krzysztof; Künzler, Markus; Mauch, Felix.
Afiliación
  • Moradi A; Department of Biology, University of Fribourg, Fribourg, Switzerland. aboubakr.moradi@unifr.ch.
  • Austerlitz T; Institute of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, Vienna, Austria.
  • Dahlin P; Agroscope, Research Division, Plant Protection, Phytopathology and Zoology in Fruit and Vegetable Production, Wädenswil, Switzerland.
  • Robert CA; Institute of Plant Sciences, University of Bern, Bern, Switzerland.
  • Maurer C; Oeschger Center for Climate Change Research, Bern, Switzerland.
  • Steinauer K; Institute of Plant Sciences, University of Bern, Bern, Switzerland.
  • van Doan C; Institute of Plant Sciences, University of Bern, Bern, Switzerland.
  • Himmighofen PA; Institute of Plant Sciences, University of Bern, Bern, Switzerland.
  • Wieczorek K; Institute of Plant Sciences, University of Bern, Bern, Switzerland.
  • Künzler M; Institute of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, Vienna, Austria.
  • Mauch F; Institute of Microbiology, Department of Biology, ETH Zürich, Zürich, Switzerland. mkuenzle@ethz.ch.
BMC Plant Biol ; 21(1): 402, 2021 Sep 01.
Article en En | MEDLINE | ID: mdl-34470613
BACKGROUND: Plant-parasitic nematodes and herbivorous insects have a significant negative impact on global crop production. A successful approach to protect crops from these pests is the in planta expression of nematotoxic or entomotoxic proteins such as crystal proteins from Bacillus thuringiensis (Bt) or plant lectins. However, the efficacy of this approach is threatened by emergence of resistance in nematode and insect populations to these proteins. To solve this problem, novel nematotoxic and entomotoxic proteins are needed. During the last two decades, several cytoplasmic lectins from mushrooms with nematicidal and insecticidal activity have been characterized. In this study, we tested the potential of Marasmius oreades agglutinin (MOA) to furnish Arabidopsis plants with resistance towards three economically important crop pests: the two plant-parasitic nematodes Heterodera schachtii and Meloidogyne incognita and the herbivorous diamondback moth Plutella xylostella. RESULTS: The expression of MOA does not affect plant growth under axenic conditions which is an essential parameter in the engineering of genetically modified crops. The transgenic Arabidopsis lines showed nearly complete resistance to H. schachtii, in that the number of female and male nematodes per cm root was reduced by 86-91 % and 43-93 % compared to WT, respectively. M. incognita proved to be less susceptible to the MOA protein in that 18-25 % and 26-35 % less galls and nematode egg masses, respectively, were observed in the transgenic lines. Larvae of the herbivorous P. xylostella foraging on MOA-expression lines showed a lower relative mass gain (22-38 %) and survival rate (15-24 %) than those feeding on WT plants. CONCLUSIONS: The results of our in planta experiments reveal a robust nematicidal and insecticidal activity of the fungal lectin MOA against important agricultural pests which may be exploited for crop protection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Aglutininas / Marasmius / Herbivoria / Nematodos Límite: Animals Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Aglutininas / Marasmius / Herbivoria / Nematodos Límite: Animals Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido