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Impact of heavy metals in the microalga Chlorella sorokiniana and assessment of its potential use in cadmium bioremediation.
León-Vaz, Antonio; León, Rosa; Giráldez, Inmaculada; Vega, José María; Vigara, Javier.
Afiliación
  • León-Vaz A; Laboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and REMSMA. University of Huelva, 210071 Huelva, Spain.
  • León R; Laboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and REMSMA. University of Huelva, 210071 Huelva, Spain.
  • Giráldez I; Department of Chemistry. Research Center in Technology of Products and Chemical Processes, PRO2TECS. University of Huelva. Campus el Carmen s/n 210071, Huelva, Spain.
  • Vega JM; Plant Biochemistry and Molecular Biology Department, Faculty of Chemistry, University of Seville, 41012 Seville, Spain.
  • Vigara J; Laboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and REMSMA. University of Huelva, 210071 Huelva, Spain. Electronic address: vigara@uhu.es.
Aquat Toxicol ; 239: 105941, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34469852
The chlorophyte microalga Chlorella sorokiniana was tested for the bioremediation of heavy metals pollution. It was cultured with different concentrations of Cu2+, Cd2+, As (III) and As (V), showing a significant inhibition on its growth at concentrations of 500 µM Cu2+, 250 µM Cd2+, 750 µM AsO33- and 5 mM AsO43- or higher. Moreover, the consumption of ammonium was also studied, showing significant differences for concentrations higher than 1 mM of Cu2+ and As (III), and 5 mM of As (V). The determination of intracellular heavy metals concentration revealed that Chlorella sorokiniana is an outstanding Cd accumulator organism, able to accumulate 11,232 mg kg-1 of Cd, and removing 65% of initial concentration of this heavy metal. Finally, antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), and enzymes involved in the production of glutamate and cysteine, such as glutamine syntethase (GS), glutamate dehydrogenase (GDH), O-acetylserine (thiol) lyase (OASTL) and NAD-isocitrate dehydrogenase (NAD-IDH) were studied both at gene expression and enzymatic activity levels. These enzymes exhibited different grades of upregulation, especially in response to Cd and As stress. However, GS expression was downregulated when Chlorella sorokiniana was cultured in the presence of these heavy metals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Chlorella / Metales Pesados / Microalgas Idioma: En Revista: Aquat Toxicol Asunto de la revista: BIOLOGIA / TOXICOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Chlorella / Metales Pesados / Microalgas Idioma: En Revista: Aquat Toxicol Asunto de la revista: BIOLOGIA / TOXICOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos