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Unraveling Toxoplasma gondii GT1 Strain Virulence and New Protein-Coding Genes with Proteogenomic Analyses.
Antil, Neelam; Kumar, Manish; Behera, Santosh Kumar; Arefian, Mohammad; Kotimoole, Chinmaya Narayana; Rex, Devasahayam Arokia Balaya; Prasad, Thottethodi Subrahmanya Keshava.
Afiliación
  • Antil N; Institute of Bioinformatics, International Technology Park, Bangalore, India.
  • Kumar M; Centre for Systems Biology and Molecular Medicine, Yenepoya Research Center, Yenepoya (Deemed to be University), Mangalore, India.
  • Behera SK; Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, India.
  • Arefian M; Institute of Bioinformatics, International Technology Park, Bangalore, India.
  • Kotimoole CN; Manipal Academy of Higher Education, Manipal, India.
  • Rex DAB; Centre for Systems Biology and Molecular Medicine, Yenepoya Research Center, Yenepoya (Deemed to be University), Mangalore, India.
  • Prasad TSK; Centre for Systems Biology and Molecular Medicine, Yenepoya Research Center, Yenepoya (Deemed to be University), Mangalore, India.
OMICS ; 25(9): 591-604, 2021 09.
Article en En | MEDLINE | ID: mdl-34468217
Toxoplasma gondii is one of the most widespread parasites of great relevance to planetary health. It infects approximately one-third of the world population. T. gondii establishes itself in warm-blooded animals and causes adverse health outcomes, particularly in immunocompromised patients. T. gondii is also widely used as a model organism to study other related apicomplexan parasites, which requires a deeper understanding of its molecular biology. Type I strains (GT1 and RH) of T. gondii are considered the most virulent forms. The whole-genome sequencing of T. gondii annotated 8460 predicted gene models in the parasite. To this end, the proteogenomics technology allows harnessing of mass spectrometry (MS)-derived proteomic data to unravel new protein-coding genes, not to mention validation and correction of the existing gene models. In this study using the proteogenomic approach, we report the identification of 31 novel protein-coding genes while reannotating 88 existing gene models. Notably, the genome annotations were corrected for genes, such as SAG5C, GRA6, ROP4, ROP5, and ROP26. The associated proteins are known to play important roles in host-parasite interactions, particularly in relation to parasite virulence, suppression of host immune response, and distinctively pertinent for the survival of the parasite inside the host system. These new findings offer new insights, informing planetary health broadly and the knowledge base on T. gondii virulence specifically. The proteogenomics approach also provides a concrete example to study related apicomplexan organisms of relevance to planetary health, and so as to develop new diagnostics and therapeutics against toxoplasmosis and related diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Proteogenómica Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: OMICS Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Proteogenómica Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: OMICS Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos