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A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Gladchuk, Alexey S; Krasnov, Konstantin A; Keltsieva, Olga A; Kalninia, Yana K; Alexandrova, Marina L; Ivanov, Nikita S; Muradymov, Marat Z; Krasnov, Nikolai V; Reynyuk, Vladimir L; Sukhodolov, Nikolai G; Podolskaya, Ekaterina P.
Afiliación
  • Gladchuk AS; Golikov Research Center of Toxicology, St. Petersburg, 192019, Russia.
  • Krasnov KA; Institute of Chemistry, St. Petersburg State University, St. Petersburg, 198504, Russia.
  • Keltsieva OA; Golikov Research Center of Toxicology, St. Petersburg, 192019, Russia.
  • Kalninia YK; Golikov Research Center of Toxicology, St. Petersburg, 192019, Russia.
  • Alexandrova ML; Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, 198095, Russia.
  • Ivanov NS; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251, Russia.
  • Muradymov MZ; Golikov Research Center of Toxicology, St. Petersburg, 192019, Russia.
  • Krasnov NV; Institute of Chemistry, St. Petersburg State University, St. Petersburg, 198504, Russia.
  • Reynyuk VL; Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, 198095, Russia.
  • Sukhodolov NG; Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, 198095, Russia.
  • Podolskaya EP; Golikov Research Center of Toxicology, St. Petersburg, 192019, Russia.
Rapid Commun Mass Spectrom ; 35(21): e9185, 2021 Nov 15.
Article en En | MEDLINE | ID: mdl-34460139
RATIONALE: The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off-line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. METHODS: A combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol-based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5-dihydroxybenzoic acid, and an n-hexane solution of polyprenols directly on a MALDI target plate. RESULTS: Peaks corresponding to [M - H + Ba]+ ions were observed in the MALDI-TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M - H + Ba]+ ions of polyprenols were discussed. CONCLUSIONS: The proposed approach can be suitable for high-throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Poliprenoles Idioma: En Revista: Rapid Commun Mass Spectrom Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Poliprenoles Idioma: En Revista: Rapid Commun Mass Spectrom Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Reino Unido