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Sequential expression of raffinose synthase and stachyose synthase corresponds to successive accumulation of raffinose, stachyose and verbascose in developing seeds of Lens culinaris Medik.
Kannan, Udhaya; Sharma, Roopam; Gangola, Manu P; Ganeshan, Seedhabadee; Båga, Monica; Chibbar, Ravindra N.
Afiliación
  • Kannan U; Department of Plant Sciences, College of Agriculture & Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
  • Sharma R; Department of Plant Sciences, College of Agriculture & Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
  • Gangola MP; Department of Plant Sciences, College of Agriculture & Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
  • Ganeshan S; Department of Plant Sciences, College of Agriculture & Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
  • Båga M; Department of Plant Sciences, College of Agriculture & Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
  • Chibbar RN; Department of Plant Sciences, College of Agriculture & Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada. Electronic address: ravi.chibbar@usask.ca.
J Plant Physiol ; 265: 153494, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34454370
Raffinose, stachyose and verbascose form the three major members of the raffinose family oligosaccharides (RFO) accumulated during seed development. Raffinose synthase (RS; EC 2.4.1.82) and stachyose synthase (STS; EC 2.4.1.67) have been associated with raffinose and stachyose synthesis, but the precise mechanism for verbascose synthesis is not well understood. In this study, full-length RS (2.7 kb) and STS (2.6 kb) clones were isolated by screening a cDNA library prepared from developing lentil seeds (18, 20, 22 and 24 days after flowering [DAF]) to understand the roles of RS and STS in RFO accumulation in developing lentil seeds. The nucleotide sequences of RS and STS genes were similar to those reported for Pisum sativum. Patterns of transcript accumulation, enzyme activities and RFO concentrations were also comparable to P. sativum. However, during lentil seed development raffinose, stachyose and verbascose accumulation corresponded to transcript accumulation for RS and STS, with peak transcript abundance occurring at about 22-24 DAF, generally followed by a sequential increase in raffinose, stachyose and verbascose concentrations followed by a steady level thereafter. Enzyme activities for RS, STS and verbascose synthase (VS) also indicated a sudden increase at around 24-26 DAF, but with an abrupt decline again coinciding with the subsequent steady state increase in the RFO. Galactan:galactan galactosyl transferase (GGT), the galactinol-independent pathway enzyme, however, exhibited steady increase in activity from 24 DAF onwards before abruptly decreasing at 34 DAF. Although GGT activity was detected, isolation of a GGT sequence from the cDNA library was not successful.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligosacáridos / Rafinosa / Semillas / Lens (Planta) / Galactosiltransferasas Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligosacáridos / Rafinosa / Semillas / Lens (Planta) / Galactosiltransferasas Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania