Your browser doesn't support javascript.
loading
A protocol for wide-scope non-target analysis of contaminants in small amounts of biota using bead beating tissuelyser extraction and LC-HRMS.
Gil-Solsona, R; Rodriguez-Mozaz, S; Diaz-Cruz, M S; Sunyer-Caldú, A; Luarte, T; Höfer, J; Galbán-Malagón, C; Gago-Ferrero, P.
Afiliación
  • Gil-Solsona R; Catalan Institute for Water Research, Girona, Spain.
  • Rodriguez-Mozaz S; Institute of Environmental Assessment and Water Research (IDAEA) Severo Ochoa Excellence Center, Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
  • Diaz-Cruz MS; University of Girona, Girona, Spain.
  • Sunyer-Caldú A; Catalan Institute for Water Research, Girona, Spain.
  • Luarte T; University of Girona, Girona, Spain.
  • Höfer J; Institute of Environmental Assessment and Water Research (IDAEA) Severo Ochoa Excellence Center, Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
  • Galbán-Malagón C; Institute of Environmental Assessment and Water Research (IDAEA) Severo Ochoa Excellence Center, Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
  • Gago-Ferrero P; Doctorado en Medicina de la Conservación, Facultad de Ciencias de la Vida, Universidad Andres Bello, Chile.
MethodsX ; 8: 101193, 2021.
Article en En | MEDLINE | ID: mdl-34434720
This work describes a robust and powerful method for wide-scope target and non-target analysis of xenobiotics in biota samples based on bead beating tissuelyser extraction, solid phase extraction (SPE) clean-up and further detection by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS). Unlike target methodologies, non-target methods usually aim at determining a wide range of still unknown substances with different physicochemical properties. Therefore, losses during the extraction process were minimised. Apart from that, the reduction of possible interferences showed to be necessary to expand the number of compounds that can be detected. This was achieved with an additional SPE clean-up step carried out with mixed-bed multi-layered cartridges. The method was validated with a set of 27 compounds covering a wide range of physicochemical properties, and further applied to the analysis of krill and fish samples.•The bead beating extraction was efficient for a wide range of organic pollutants in small quantities of biota samples.•Multi-layered solid phase extraction clean-up yield a wide xenobiotics coverage reducing matrix effects.•Method validation with 27 compounds led to a suitable method for non-target analysis of organic pollutants in biota.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: MethodsX Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: MethodsX Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos