The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum.

Paradis, F W; Warren, R A; Kilburn, D G; Miller, R C

Paradis, F W; Warren, R A; Kilburn, D G; Miller, R C.

Afiliación

Paradis FW; Department of Microbiology, University of British Columbia, Vancouver, Canada.

Gene ; 61(2): 199-206, 1987.

Article en En | MEDLINE | ID: mdl-3443308

RESUMEN

The exoglucanase gene (cex) and the endoglucanase A gene (cenA) from Cellulomonas fimi were subcloned into the Escherichia coli/Brevibacterium lactofermentum shuttle vector pBK10. Both genes were expressed to five to ten times higher levels in B. lactofermentum than in E. coli, probably because these genes were expressed from C. fimi promoters. In B. lactofermentum virtually all of the enzyme activities were in the culture supernatant. This system will facilitate analysis of the expression of the C. fimi genes in and secretion of their products from a Gram-positive bacterium.

Asunto(s)

Brevibacterium/genética; Celulasa/genética; Bacterias Grampositivas/genética; Clonación Molecular; Regulación de la Expresión Génica; Genes Bacterianos; Vectores Genéticos; Bacterias Grampositivas/enzimología; Transformación Genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brevibacterium / Celulasa / Bacterias Grampositivas Idioma: En Revista: Gene Año: 1987 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos

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