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Diagnostic Usefulness of MLPA Techniques for Recurrent Copy Number Variants Detection in Global Developmental Delay/Intellectual Disability.
Miclea, Diana; Szucs, Adriana; Mirea, Andreea; Stefan, Delia-Maria; Nazarie, Florina; Bucerzan, Simona; Lazea, Cecilia; Grama, Alina; Pop, Tudor Lucian; Farcas, Marius; Zaharie, Gabriela; Matyas, Melinda; Mager, Monica; Vintan, Mihaela; Popp, Radu; Alkhzouz, Camelia.
Afiliación
  • Miclea D; Department of Molecular Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Szucs A; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Mirea A; Department of Molecular Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Stefan DM; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Nazarie F; Department of Molecular Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Bucerzan S; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Lazea C; Department of Molecular Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Grama A; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Pop TL; Department of Molecular Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Farcas M; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Zaharie G; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Matyas M; Department of Mother and Child, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Mager M; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Vintan M; Department of Mother and Child, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
  • Popp R; Emergency Clinical Hospital for Children, Cluj-Napoca, Romania.
  • Alkhzouz C; Department of Mother and Child, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Int J Gen Med ; 14: 4511-4515, 2021.
Article en En | MEDLINE | ID: mdl-34429637
BACKGROUND: Genetic testing has become a standardized practice in the diagnosis of patients with global developmental delay/intellectual disability (GDD/ID). The aim of this study is to observe the frequency of recurrent copy number variations (CNVs) in patients diagnosed with GDD/ID, using MLPA technique. METHODS: A total of 501 paediatric patients with GDD/ID were analysed using SALSA MLPA probemix P245 Microdeletion Syndromes-1A, and the technical steps were performed according to the MRC Holland MLPA general protocol. RESULTS: Twenty-five of 501 patients (5%) were diagnosed with a microdeletion/microduplication syndrome. Amongst them, 7 of 25 (30%) with clinical suggestion have a confirmed diagnosis, for the other cases the clinical features were not evocative for a specific syndrome. CONCLUSION: This study showed that in cases with a specific clinical diagnosis the MLPA technique could be a useful alternative, less expensive and more efficient to indicate as first intention of a targeted diagnostic test, as it is the case of Williams syndrome, Prader-Willi syndrome or DiGeorge syndrome.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: Int J Gen Med Año: 2021 Tipo del documento: Article País de afiliación: Rumanía Pais de publicación: Nueva Zelanda

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: Int J Gen Med Año: 2021 Tipo del documento: Article País de afiliación: Rumanía Pais de publicación: Nueva Zelanda