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Curcumol inhibits EBV-positive Nasopharyngeal carcinoma migration and invasion by targeting nucleolin.
Guan, Xiao; Yu, Dan; HuangFu, Mengjie; Huang, Zhiyi; Dou, Tong; Liu, Yisa; Zhou, Luwei; Li, Xumei; Wang, Lin; Liu, Haiping; Wang, Juan; Chen, Xu.
Afiliación
  • Guan X; Xiangya Hospital, Central South University, Changsha 410008, China.
  • Yu D; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • HuangFu M; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • Huang Z; Pathology Department, Affiliated Hospital of Guilin Medical University, Guilin 541001, China.
  • Dou T; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • Liu Y; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • Zhou L; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • Li X; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • Wang L; College of Pharmacy, Guilin Medical University, Guilin 541199, China.
  • Liu H; Science and Technology Department, Guilin Medical University, Guilin 541199, China.
  • Wang J; Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541199, China. Electronic address: juanlovelife@163.com.
  • Chen X; College of Pharmacy, Guilin Medical University, Guilin 541199, China. Electronic address: chenxu@glmc.edu.cn.
Biochem Pharmacol ; 192: 114742, 2021 10.
Article en En | MEDLINE | ID: mdl-34428442
Metastasis is a major cause of recurrence and death in patients with EBV-positive Nasopharyngeal carcinoma (NPC). Previous reports documented that curcumol has both anti-cancer and anti-viral effects, but there is little literature systematically addressing the mechanism of curcumol in EBV-positive tumors. Previously we found that nucelolin (NCL) is a target protein of curcumol in CNE2 cells, an EBV-negative NPC, and in this experiment, we reported a critical role for NCL in promoting migration and invasion of C666-1 cells, an EBV-positive NPC, and found that the expression of NCL determined the level of curcumol's efficacy. Mechanistically, NCL interacted with Epstein-Barr Virus Nuclear Antigen 1 (EBNA1) to activate VEGFA/VEGFR1/PI3K/AKT signaling pathway, which in turn promoted NPC cell invasion and metastasis. Moreover, further study showed that the differential expression of NCL and curcumol intervention only had a regulatory effect on the nuclear accumulation of VEGFR1, which strengthened the anti-cancer effect of curcumol mediated through NCL. Our findings indicated that curcumol exerted anti EBV-positive NPC invasion and metastasis by downregulating EBNA1 and inhibiting VEGFA/VEGFR1/PI3K/AKT signaling by targeting NCL, which provides a novel pharmacological basis for curcumol's clinical use in treating patients with EBV-positive NPC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Medicamentos Herbarios Chinos / Movimiento Celular / Neoplasias Nasofaríngeas / Herpesvirus Humano 4 / Carcinoma Nasofaríngeo Límite: Animals / Humans Idioma: En Revista: Biochem Pharmacol Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Medicamentos Herbarios Chinos / Movimiento Celular / Neoplasias Nasofaríngeas / Herpesvirus Humano 4 / Carcinoma Nasofaríngeo Límite: Animals / Humans Idioma: En Revista: Biochem Pharmacol Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido