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Phosphorylation of CrkL S114 induced by common gamma chain cytokines and T-cell receptor signal transduction.
Estrada, Armando; Rodriguez, Alejandro C; Rodriguez, Georgialina; Grant, Alice H; Ayala-Marin, Yoshira M; Arrieta, Amy J; Kirken, Robert A.
Afiliación
  • Estrada A; Department of Biological Sciences, The University of Texas At El Paso, El Paso, TX, 79968, USA.
  • Rodriguez AC; Border Biomedical Research Center, The University of Texas At El Paso, El Paso, TX, 79968, USA.
  • Rodriguez G; Department of Biological Sciences, The University of Texas At El Paso, El Paso, TX, 79968, USA.
  • Grant AH; Border Biomedical Research Center, The University of Texas At El Paso, El Paso, TX, 79968, USA.
  • Ayala-Marin YM; Department of Biological Sciences, The University of Texas At El Paso, El Paso, TX, 79968, USA.
  • Arrieta AJ; Border Biomedical Research Center, The University of Texas At El Paso, El Paso, TX, 79968, USA.
  • Kirken RA; Department of Biological Sciences, The University of Texas At El Paso, El Paso, TX, 79968, USA.
Sci Rep ; 11(1): 16951, 2021 08 20.
Article en En | MEDLINE | ID: mdl-34417497
T-cell activation and cellular expansion by common gamma chain cytokines such as Interleukin-2 is necessary for adaptive immunity. However, when unregulated these same pathways promote pathologies ranging from autoimmune disorders to cancer. While the functional role of Interleukin-2 and downstream effector molecules is relatively clear, the repertoire of phosphoregulatory proteins downstream of this pathway is incomplete. To identify phosphoproteins downstream of common gamma chain receptor, YT cells were radiolabeled with [32P]-orthophosphate and stimulated with Interleukin-2. Subsequently, tyrosine phosphorylated proteins were immunopurified and subjected to tandem mass spectrometry-leading to the identification of CrkL. Phosphoamino acid analysis revealed concurrent serine phosphorylation of CrkL and was later identified as S114 by mass spectrometry analysis. S114 was inducible through stimulation with Interleukin-2 or T-cell receptor stimulation. Polyclonal antibodies were generated against CrkL phospho-S114, and used to show its inducibility by multiple stimuli. These findings confirm CrkL as an Interleukin-2 responsive protein that becomes phosphorylated at S114 by a kinase/s downstream of PI3K and MEK/ERK signaling.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoserina / Receptores de Antígenos de Linfocitos T / Transducción de Señal / Citocinas / Interleucina-2 / Proteínas Adaptadoras Transductoras de Señales / Subunidad gamma Común de Receptores de Interleucina Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoserina / Receptores de Antígenos de Linfocitos T / Transducción de Señal / Citocinas / Interleucina-2 / Proteínas Adaptadoras Transductoras de Señales / Subunidad gamma Común de Receptores de Interleucina Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido