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Regional disturbance of the distribution of T regulatory cells and T helper cells associated with irregular-shaped germinal centers in immunoglobulin G4-related sialadenitis.
Kasashima, Satomi; Kawashima, Atsuhiro; Kurose, Nozomu; Ozaki, Satoru; Ikeda, Hiroko; Harada, Ken-Ichi.
Afiliación
  • Kasashima S; Department of Clinical Laboratory Science, Graduate School of Health Science, Kanazawa University, Kanazawa, Japan. sato-kasa@staff.kanazawa-u.ac.jp.
  • Kawashima A; Department of Pathology, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Japan. sato-kasa@staff.kanazawa-u.ac.jp.
  • Kurose N; Department of Clinical Laboratory, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Japan. sato-kasa@staff.kanazawa-u.ac.jp.
  • Ozaki S; Department of Pathology, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Japan.
  • Ikeda H; Department of Clinical Laboratory, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Japan.
  • Harada KI; Department of Pathology, National Hospital Organization, Kanazawa Medical Center, Kanazawa, Japan.
Virchows Arch ; 479(6): 1221-1232, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34415430
Although many germinal centers (GCs) have been reported in immunoglobulin (Ig) G4-related disease, the significance of GCs in IgG4-related disease has not received attention. Both T follicular regulatory cells (Tfr), which are regulatory T cells (Treg) in GCs, and T follicular helper cells (Tfh) produce the cytokine interleukin (IL)-10 and regulate GC development. In whole-slide image analysis in surgical specimens using immunohistochemistry, IgG4-related sclerosing sialadenitis (IgG4-SS, n = 17) was characterized by markedly numerous, large, and irregular-shaped GCs with increased IL-10 + cells and Tfr and Tfh in the total area of the salivary gland compared with controls, including patients with chronic sialadenitis (n = 17) and Sjögren syndrome (n = 15). In particular, the central area of GC in IgG4-SS showed a higher Tfr number and Tfr/Tfh ratio than controls. The number of Tfr in the central area was significantly correlated with the number of IgG4 + plasmacytes and the number, size, and irregularity of GCs. In the mantle area, which surrounds GCs, IgG4-SS showed a higher Treg number and Treg/T helper cells (Th) ratio than controls. In IgG4-SS, the Treg/Th ratio was highest in the mantle area outside GCs and the Tfr/Tfh ratio was highest in the central area inside GCs. However, in controls, the Treg/Th ratio gradually decreased from outside to inside GCs. Our findings reveal that the morphological abnormality of GCs and the characteristic localization and altered balance of Treg and Th in the different compartments of inside and outside GCs would be the novel hallmarks of IgG4-SS.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glándulas Salivales / Sialadenitis / Linfocitos T Reguladores / Centro Germinal / Estructuras Linfoides Terciarias / Enfermedad Relacionada con Inmunoglobulina G4 / Células T Auxiliares Foliculares Tipo de estudio: Diagnostic_studies / Observational_studies / Risk_factors_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Virchows Arch Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glándulas Salivales / Sialadenitis / Linfocitos T Reguladores / Centro Germinal / Estructuras Linfoides Terciarias / Enfermedad Relacionada con Inmunoglobulina G4 / Células T Auxiliares Foliculares Tipo de estudio: Diagnostic_studies / Observational_studies / Risk_factors_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Virchows Arch Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania