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Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology.
Cortella, Lucas R X; Cestari, Idágene A; Lahuerta, Ricardo D; Araña, Matheus C; Soldera, Marcos; Rank, Andreas; Lasagni, Andrés F; Cestari, Ismar N.
Afiliación
  • Cortella LRX; Bioengineering Department, Heart Institute (InCor), University of São Paulo Medical School, Av. Dr Enéas de Carvalho Aguiar, 44, 05403-900 São Paulo, Brazil.
  • Cestari IA; Bioengineering Department, Heart Institute (InCor), University of São Paulo Medical School, Av. Dr Enéas de Carvalho Aguiar, 44, 05403-900 São Paulo, Brazil.
  • Lahuerta RD; Bioengineering Department, Heart Institute (InCor), University of São Paulo Medical School, Av. Dr Enéas de Carvalho Aguiar, 44, 05403-900 São Paulo, Brazil.
  • Araña MC; Bioengineering Department, Heart Institute (InCor), University of São Paulo Medical School, Av. Dr Enéas de Carvalho Aguiar, 44, 05403-900 São Paulo, Brazil.
  • Soldera M; Institute for Manufacturing Technology, Technische Universität Dresden, George-Baehr-Str. 3c, 01069 Dresden, Germany.
  • Rank A; PROBIEN-CONICET, Dto. de Electrotecnia, Universidad Nacional del Comahue, Buenos Aires 1400, 8300 Neuquén, Argentina.
  • Lasagni AF; Institute for Manufacturing Technology, Technische Universität Dresden, George-Baehr-Str. 3c, 01069 Dresden, Germany.
  • Cestari IN; Institute for Manufacturing Technology, Technische Universität Dresden, George-Baehr-Str. 3c, 01069 Dresden, Germany.
Biomed Mater ; 16(6)2021 09 07.
Article en En | MEDLINE | ID: mdl-34412045
Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 µm on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Nanotecnología / Miocitos Cardíacos / Células Madre Pluripotentes Inducidas Límite: Humans Idioma: En Revista: Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Nanotecnología / Miocitos Cardíacos / Células Madre Pluripotentes Inducidas Límite: Humans Idioma: En Revista: Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido