Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy.
STAR Protoc
; 2(3): 100718, 2021 09 17.
Article
en En
| MEDLINE
| ID: mdl-34401784
FACS sorting followed by single-cell RNA-sequencing (SORT-Seq) is a popular procedure to select cells of interest for single-cell transcriptomics. However, FACS is not suitable for measurement of subcellular distribution of fluorescence or for small samples (<1,000 cells). The VYCAP puncher system overcomes these limitations. Here, we describe a workflow to capture, image, and collect fluorescent human retina pigment epithelium cells for SORT-Seq using this system. The workflow can be used for any cell type with a diameter of â¼5-50 µm. For complete details on the use and execution of this protocol, please refer to Segeren et al. (2020).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Separación Celular
/
Análisis de la Célula Individual
/
Microscopía Fluorescente
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Estados Unidos