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Enzyme-based sensing on nanohybrid film coated over FTO electrode for highly sensitive detection of antibiotics.
Chauhan, Nidhi; Balayan, Sapna; Gupta, Shaivya; Singh, Jaskaran; Jain, Utkarsh.
Afiliación
  • Chauhan N; Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida, 201313, India.
  • Balayan S; Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida, 201313, India.
  • Gupta S; Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida, 201313, India.
  • Singh J; School of Allied Health Sciences (SAHS), Sharda University, Greater Noida, 201306, Uttar Pradesh, India.
  • Jain U; Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida, 201313, India. karshjain@gmail.com.
Bioprocess Biosyst Eng ; 44(12): 2469-2479, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34386846
Cefepime and Meropenem are the new class of antibiotics, which are particularly used as last potent defender or the antibiotics of the last resort against multi-resistant bacterial species. In this paper, an impedance-based electrochemical biosensor was fabricated for identifying antibiotics of last resort in the forensic samples including gastric lavage and other body fluids. The sensor was developed using platinum nanoparticles (PtNPs) and electrodeposited zinc oxide- zinc hexacyanoferrate hybrid film (ZnO/ZnHCF) on the surface of a fluorine-doped glass electrode (FTO). Further, penicillinase was immobilized onto the modified electrode using penicillinase enzyme. The developed biosensor exhibits a good analytical response for the detection of antibiotics evaluated using electrochemistry studies. The linear response of the fabricated electrode was observed from 0.1 to 750 µM and the electrode limit of detection (LOD) was observed as 0.1 µM. The sensor confirms good accuracy, is highly selective, and sensitive for the target. While storing the modified electrode at 4 °C, the stability of biosensor was evaluated for 45 days, and activity loss of 30-40% was observed. The highly sensitive interface of Penicillinase@CHIT/PtNP-ZnO/ZnHCF/FTO electrode shows a promising future in forensic studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicilinasa / Platino (Metal) / Óxido de Zinc / Electrodos / Nanopartículas del Metal / Ferrocianuros / Flúor / Antibacterianos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicilinasa / Platino (Metal) / Óxido de Zinc / Electrodos / Nanopartículas del Metal / Ferrocianuros / Flúor / Antibacterianos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania