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Morphological features of breast cancer circulating tumor cells in blood after physical and biological type of isolation.
Jesenko, Tanja; Modic, Ziva; Kuhar, Cvetka Grasic; Cemazar, Maja; Matkovic, Urska; Miceska, Simona; Varl, Jerneja; Kuhar, Anamarija; Kloboves-Prevodnik, Veronika.
Afiliación
  • Jesenko T; Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
  • Modic Z; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
  • Kuhar CG; Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
  • Cemazar M; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
  • Matkovic U; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
  • Miceska S; Department of Medical Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
  • Varl J; Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
  • Kuhar A; Faculty of Health Sciences, University of Primorska, Izola, Slovenia.
  • Kloboves-Prevodnik V; Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
Radiol Oncol ; 55(3): 292-304, 2021 08 10.
Article en En | MEDLINE | ID: mdl-34384011
BACKGROUND: Circulating tumor cells (CTCs) have become an important biomarker in breast cancer. Different isolation tech-niques based on their biological or physical features were established. Currently, the most widely used methods for visualization after their separation are based on immunofluorescent staining, which does not provide the information on the morphology. MATERIALS AND METHODS: The aim of this study was to evaluate how two different separation techniques affect cell morphology and to analyse cell morphology with techniques used in routine cytopathological laboratory. A direct side-by-side comparison of physical (Parsortix®) and biological (MACS®) separation technique was performed. RESULTS: In the preclinical setting, both isolation techniques retained the viability and antigenic characteristics of MCF7 breast cancer cells. Some signs of degeneration such as cell swelling, cytoplasmic blebs, villous projections and vacuolization were observed. In metastatic breast cancer patient cohort, morphological features of isolated CTCs were dependent on the separation technique. After physical separation, CTCs with preserved cell morphology were detected. After biological separation the majority of the isolated CTCs were so degenerated that their identity was difficult to confirm. CONCLUSIONS: Taken together, physical separation is a suitable technique for detection of CTCs with preserved cell morphology for the use in a routine cytopathological laboratory.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Separación Celular / Forma de la Célula / Células Neoplásicas Circulantes Límite: Female / Humans Idioma: En Revista: Radiol Oncol Año: 2021 Tipo del documento: Article País de afiliación: Eslovenia Pais de publicación: Polonia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Separación Celular / Forma de la Célula / Células Neoplásicas Circulantes Límite: Female / Humans Idioma: En Revista: Radiol Oncol Año: 2021 Tipo del documento: Article País de afiliación: Eslovenia Pais de publicación: Polonia