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Perfluoroalkyl substances are increased in patients with late-onset ulcerative colitis and induce intestinal barrier defects ex vivo in murine intestinal tissue.
Fart, Frida; Salihovic, Samira; McGlinchey, Aidan; Gareau, Melanie G; Oresic, Matej; Halfvarson, Jonas; Hyötyläinen, Tuulia; Schoultz, Ida.
Afiliación
  • Fart F; School of Medical Sciences, Örebro University, Örebro, Sweden.
  • Salihovic S; School of Medical Sciences, Örebro University, Örebro, Sweden.
  • McGlinchey A; School of Science and Technology, Örebro University, Örebro, Sweden.
  • Gareau MG; School of Medical Sciences, Örebro University, Örebro, Sweden.
  • Oresic M; Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis, Davis, CA, USA.
  • Halfvarson J; School of Medical Sciences, Örebro University, Örebro, Sweden.
  • Hyötyläinen T; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland.
  • Schoultz I; Department of Gastroenterology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Scand J Gastroenterol ; 56(11): 1286-1295, 2021 11.
Article en En | MEDLINE | ID: mdl-34383611
BACKGROUND: Environmental factors are strongly implicated in late-onset of inflammatory bowel disease. Here, we investigate whether high levels of perfluoroalkyl substances are associated with (1) late-onset inflammatory bowel disease, and (2) disturbances of the bile acid pool. We further explore the effect of the specific perfluoroalkyl substance perfluorooctanoic acid on intestinal barrier function in murine tissue. METHODS: Serum levels of perfluoroalkyl substances and bile acids were assessed by ultra-performance liquid chromatography coupled to a triple-quadrupole mass spectrometer in matched samples from patients with ulcerative colitis (n = 20) and Crohn's disease (n = 20) diagnosed at the age of ≥55 years. Age and sex-matched blood donors (n = 20), were used as healthy controls. Ex vivo Ussing chamber experiments were performed to assess the effect of perfluorooctanoic acid on ileal and colonic murine tissue (n = 9). RESULTS: The total amount of perfluoroalkyl substances was significantly increased in patients with ulcerative colitis compared to healthy controls and patients with Crohn's disease (p < .05). Ex vivo exposure to perfluorooctanoic acid induced a significantly altered ileal and colonic barrier function. The distribution of bile acids, as well as the correlation pattern between (1) perfluoroalkyl substances and (2) bile acids, differed between patient and control groups. DISCUSSION: Our results demonstrate that perfluoroalkyl substances levels are increased in patients with late-onset ulcerative colitis and may contribute to the disease by inducing a dysfunctional intestinal barrier.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Inflamatorias del Intestino / Colitis Ulcerosa / Enfermedad de Crohn / Fluorocarburos Límite: Animals / Humans / Middle aged Idioma: En Revista: Scand J Gastroenterol Año: 2021 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Inflamatorias del Intestino / Colitis Ulcerosa / Enfermedad de Crohn / Fluorocarburos Límite: Animals / Humans / Middle aged Idioma: En Revista: Scand J Gastroenterol Año: 2021 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido