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Microscopic impedance cytometry for quantifying single cell shape.
Tang, Tao; Liu, Xun; Kiya, Ryota; Shen, Yigang; Yuan, Yapeng; Zhang, Tianlong; Suzuki, Kengo; Tanaka, Yo; Li, Ming; Hosokawa, Yoichiroh; Yalikun, Yaxiaer.
Afiliación
  • Tang T; Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan.
  • Liu X; Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan.
  • Kiya R; Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan.
  • Shen Y; Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka Suita, Osaka, 565-0871, Japan.
  • Yuan Y; Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka Suita, Osaka, 565-0871, Japan.
  • Zhang T; Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan; School of Engineering, Macquarie University, Sydney, 2109, Australia.
  • Suzuki K; Euglena Co. Ltd., Tokyo 108-0014, Japan.
  • Tanaka Y; Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka Suita, Osaka, 565-0871, Japan.
  • Li M; School of Engineering, Macquarie University, Sydney, 2109, Australia.
  • Hosokawa Y; Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan.
  • Yalikun Y; Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan; Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka Suita, Osaka, 565-0871, Japan. Electronic address: yaxiaer@ms.naist.jp.
Biosens Bioelectron ; 193: 113521, 2021 Dec 01.
Article en En | MEDLINE | ID: mdl-34380102
In this work, we investigated the ability of impedance flow cytometry to measure the shape of single cells/particles. We found that the impedance pulses triggered by micro-objects that are asymmetric in morphology show a tilting trend, and there is no such a tilting trend for symmetric ones. Therefore, we proposed a new metric, tilt index, to quantify the tilt level of the impedance pulses. Through simulation, we found that the value of tilt index tends to be zero for perfectly symmetrical objects, while the value is greater than zero for asymmetrical ones. Also, this metric was found to be independent on the trajectories (i.e., lateral, and z-direction shift) of the target micro-object. In experiments, we adopted a home-made lock-in amplifier and performed experiments on 10 µm polystyrene beads and Euglena gracilis (E. gracilis) cells with varying shapes. The experimental results coincided with the simulation results and demonstrated that the new metric (tilt index) enables the impedance cytometry to characterize the shape single cells/particles without microscopy or other optical setups.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido