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Immobilization of papain: A review.
Tacias-Pascacio, Veymar G; Morellon-Sterling, Roberto; Castañeda-Valbuena, Daniel; Berenguer-Murcia, Ángel; Kamli, Majid Rasool; Tavano, Olga; Fernandez-Lafuente, Roberto.
Afiliación
  • Tacias-Pascacio VG; Facultad de Ciencias de la Nutrición y Alimentos, Universidad de Ciencias y Artes de Chiapas, Lib. Norte Pte. 1150, 29039 Tuxtla Gutiérrez, Chiapas, Mexico; Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, 29050 Tuxtla Gutiérrez, Chiapas, Mex
  • Morellon-Sterling R; Departamento de Biocatálisis. ICP-CSIC./Marie Curie 2, Campus UAM-CSIC Cantoblanco, 28049 Madrid. Spain; Student of Departamento de Biología Molecular, Universidad Autónoma de Madrid, Darwin 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid. Spain.
  • Castañeda-Valbuena D; Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, 29050 Tuxtla Gutiérrez, Chiapas, Mexico.
  • Berenguer-Murcia Á; Departamento de Química Inorgánica e Instituto Universitario de Materiales, Universidad de Alicante, Alicante, Spain.
  • Kamli MR; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddad 21589, Saudi Arabia; Center of excellence in Bionanoscience Research, King Abdulaziz University, Jeddad 21589, Saudi Arabia.
  • Tavano O; Faculty of Nutrition, Alfenas Federal Univ., 700 Gabriel Monteiro da Silva St, Alfenas, MG 37130-000, Brazil.
  • Fernandez-Lafuente R; Departamento de Biocatálisis. ICP-CSIC./Marie Curie 2, Campus UAM-CSIC Cantoblanco, 28049 Madrid. Spain; Center of Excellence in Bionanoscience Research, External advisory board, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Electronic address: rfl@icp.csic.es.
Int J Biol Macromol ; 188: 94-113, 2021 Oct 01.
Article en En | MEDLINE | ID: mdl-34375660
Papain is a cysteine protease from papaya, with many applications due to its broad specificity. This paper reviews for first time the immobilization of papain on different supports (organic, inorganic or hybrid supports) presenting some of the features of the utilized immobilization strategies (e.g., epoxide, glutaraldehyde, genipin, glyoxyl for covalent immobilization). Special focus is placed on the preparation of magnetic biocatalysts, which will permit the simple recovery of the biocatalyst even if the medium is a suspension. Problems specific to the immobilization of proteases (e.g., steric problems when hydrolyzing large proteins) are also defined. The benefits of a proper immobilization (enzyme stabilization, widening of the operation window) are discussed, together with some artifacts that may suggest an enzyme stabilization that may be unrelated to enzyme rigidification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papaína / Carica / Enzimas / Enzimas Inmovilizadas Idioma: En Revista: Int J Biol Macromol Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papaína / Carica / Enzimas / Enzimas Inmovilizadas Idioma: En Revista: Int J Biol Macromol Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos