STATEMENT OF PROBLEM: Information regarding three-dimensional-printed (3D-printed) dentures, especially when using the additive manufacturing technique, and the repair strength of this type of denture is sparse. PURPOSE: The purpose of this in vitro study was to assess the effect of different surface treatments on the surface roughness and flexural strength of repaired 3D-printed denture base. MATERIAL AND METHODS: One hundred and twenty 3D-printed bar-shaped specimens were fabricated from acrylic resin and divided into 6 groups (n=20). The positive control group consisted of intact specimens. The other specimens were sectioned in half with a 1-mm gap. Except for the specimens in the negative control group, the remaining specimens were treated with erbium: yttrium-aluminum-garnet (Er:YAG) laser, airborne-particle abrasion, a combination of laser and airborne-particle abrasion, and bur grinding. All sectioned specimens were repaired by autopolymerizing acrylic resin and thermocycled after measuring their surface roughness with a profilometer. The flexural strength test was performed with a universal testing machine. One specimen of each group was inspected under a scanning electron microscope. The data were analyzed with ANOVA, followed by the Games-Howell post hoc test or the Kruskal-Wallis test followed by the Mann-Whitney test with Bonferroni adjustment. RESULTS: The mean flexural strength of the PC group was significantly higher than that of all repaired groups (P<.001). All surface-treated groups showed significantly higher flexural strength (P<.05) and surface roughness (P<.004) than the negative control group. Bur grinding provided significantly higher flexural strength than other surface treatments (P<.001) and higher surface roughness than laser and airborne-particle abrasion plus laser (P<.001). CONCLUSIONS: All surface treatments significantly increased the surface roughness and flexural strength, but none of them yielded a strength comparable with that of the intact group. Bur grinding provided the highest flexural strength.