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High cell density cultivation of Corynebacterium glutamicum on bio-based lignocellulosic acetate using pH-coupled online feeding control.
Kiefer, Dirk; Merkel, Manuel; Lilge, Lars; Hausmann, Rudolf; Henkel, Marius.
Afiliación
  • Kiefer D; University of Hohenheim, Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, Fruwirthstrasse 12, 70599 Stuttgart, Germany.
  • Merkel M; University of Hohenheim, Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, Fruwirthstrasse 12, 70599 Stuttgart, Germany.
  • Lilge L; University of Hohenheim, Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, Fruwirthstrasse 12, 70599 Stuttgart, Germany.
  • Hausmann R; University of Hohenheim, Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, Fruwirthstrasse 12, 70599 Stuttgart, Germany.
  • Henkel M; University of Hohenheim, Institute of Food Science and Biotechnology, Department of Bioprocess Engineering, Fruwirthstrasse 12, 70599 Stuttgart, Germany. Electronic address: Marius.Henkel@uni-hohenheim.de.
Bioresour Technol ; 340: 125666, 2021 Nov.
Article en En | MEDLINE | ID: mdl-34352645
Acetate represents a promising alternative carbon source for future industrial biotechnology. In this study, the high potential of Corynebacterium glutamicum for utilizing acetate as sole carbon source was demonstrated. Batch culture studies revealed that C. glutamicum ATCC 13032 naturally exhibits high acetate tolerance with maximum growth rates (µmax = 0.47 h-1) similar to those on D-glucose. Based on a simple and auto-regulated pH-coupled feeding strategy which utilizes bio-acetic acid in pure form, a novel and high-efficient fed-batch process was developed in a 42 L stirred-tank bioreactor. By optimizing the carbon-to-nitrogen (C/N) feeding ratio, maximum biomass concentrations of 80.2 gCDW/L were achieved with a space-time yield of 66.6 gCDW/L·d. In addition, a process model was implemented describing the time-courses of biomass growth and substrate concentrations. This is the first study in which an industrial platform organism was grown to high cell densities using green, lignocellulosic acetate as an alternative carbon source.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Corynebacterium glutamicum Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Corynebacterium glutamicum Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido