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Nanocellulose for peripheral nerve regeneration in rabbits using citric acid as crosslinker with chitosan and freeze/thawed PVA.
de Lima, Gabriel G; Júnior, Emílio L de S; Aggio, Bruno B; Shee, Bor Shin; Filho, Emanuel M de M; Segundo, Francisco A de S; Fournet, Margaret B; Devine, Declan M; Magalhães, Washington L E; de Sá, Marcelo J C.
Afiliación
  • de Lima GG; Programa de Pós-Graduação em Engenharia e Ciência dos Materiais-PIPE, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Júnior ELS; Materials Research Institute, Athlone Institute of Technology, Athlone, Ireland.
  • Aggio BB; Programa de Pós-Graduação em Medicina Veterinária-PPGMV, Universidade Federal de Campina Grande, Campina Grande PB, Brazil.
  • Shee BS; Departamento de Química, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
  • Filho EMM; Materials Research Institute, Athlone Institute of Technology, Athlone, Ireland.
  • Segundo FAS; Programa de Pós-Graduação em Medicina Veterinária-PPGMV, Universidade Federal de Campina Grande, Campina Grande PB, Brazil.
  • Fournet MB; Programa de Pós-Graduação em Medicina Veterinária-PPGMV, Universidade Federal de Campina Grande, Campina Grande PB, Brazil.
  • Devine DM; Materials Research Institute, Athlone Institute of Technology, Athlone, Ireland.
  • Magalhães WLE; Materials Research Institute, Athlone Institute of Technology, Athlone, Ireland.
  • de Sá MJC; Programa de Pós-Graduação em Engenharia e Ciência dos Materiais-PIPE, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Biomed Mater ; 16(5)2021 08 13.
Article en En | MEDLINE | ID: mdl-34330112
This work investigates peripheral nerve regeneration using membranes consisting of pure chitosan (CHI), which was further blended with nanofibrillated cellulose, with citric acid as crosslinker, with posterior addition of polyvinyl alcohol, with subsequent freeze thawing. Nanocellulose improves the mechanical and thermal resistance, as well as flexibility of the film, which is ideal for the surgical procedure. The hydrogel presented a slow rate of swelling, which is adequate for cell and drug delivery. A series ofin vitrotests revealed to be non-toxic for neuronal Schwann cell from the peripheral nervous system of Rattus norvegicus, while there was a slight increase in toxicity if crosslink is performed-freeze-thaw. Thein vivoresults, using rabbits with a 5 mm gap nerve defect, revealed that even though pure CHI was able to regenerate the nerve, it did not present functional recovery with only the deep pain attribute being regenerated. When autologous implant was used jointly with the biomaterial membrane, as a covering agent, it revealed a functional recovery within 15 d when cellulose and the hydrogel were introduced, which was attributed to the film charge interaction that may help influence the neuronal axons growth into correct locations. Thus, indicating that this system presents ideal regeneration as nerve conduits.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Celulosa / Ácido Cítrico / Quitosano / Nanofibras / Regeneración Nerviosa Límite: Animals Idioma: En Revista: Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Celulosa / Ácido Cítrico / Quitosano / Nanofibras / Regeneración Nerviosa Límite: Animals Idioma: En Revista: Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido