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Extension of Collagen Deposition in COVID-19 Post Mortem Lung Samples and Computed Tomography Analysis Findings.
Ball, Lorenzo; Barisione, Emanuela; Mastracci, Luca; Campora, Michela; Costa, Delfina; Robba, Chiara; Battaglini, Denise; Micali, Marco; Costantino, Federico; Cittadini, Giuseppe; Patroniti, Nicolò; Pelosi, Paolo; Fiocca, Roberto; Grillo, Federica.
Afiliación
  • Ball L; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
  • Barisione E; Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Mastracci L; Interventional Pulmonology Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Campora M; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
  • Costa D; Anatomic Pathology Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Robba C; Surgical Pathology Unit, Santa Chiara Hospital, Provincial Agency for Health Services, 38122 Trento, Italy.
  • Battaglini D; Molecular Oncology and Angiogenesis Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Micali M; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
  • Costantino F; Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Cittadini G; Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Patroniti N; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
  • Pelosi P; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
  • Fiocca R; Radiology Department, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.
  • Grillo F; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Int J Mol Sci ; 22(14)2021 Jul 13.
Article en En | MEDLINE | ID: mdl-34299124
Lung fibrosis has specific computed tomography (CT) findings and represents a common finding in advanced COVID-19 pneumonia whose reversibility has been poorly investigated. The aim of this study was to quantify the extension of collagen deposition and aeration in postmortem cryobiopsies of critically ill COVID-19 patients and to describe the correlations with qualitative and quantitative analyses of lung CT. Postmortem transbronchial cryobiopsy samples were obtained, formalin fixed, paraffin embedded and stained with Sirius red to quantify collagen deposition, defining fibrotic samples as those with collagen deposition above 10%. Lung CT images were analyzed qualitatively with a radiographic score and quantitatively with computer-based analysis at the lobe level. Thirty samples from 10 patients with COVID-19 pneumonia deceased during invasive mechanical ventilation were included in this study. The median [interquartile range] percent collagen extension was 6.8% (4.6-16.2%). In fibrotic compared to nonfibrotic samples, the qualitative score was higher (260 (250-290) vs. 190 (120-270), p = 0.036) while the gas fraction was lower (0.46 (0.32-0.47) vs. 0.59 (0.37-0.68), p = 0.047). A radiographic score above 230 had 100% sensitivity (95% confidence interval, CI: 66.4% to 100%) and 66.7% specificity (95% CI: 41.0% to 92.3%) to detect fibrotic samples, while a gas fraction below 0.57 had 100% sensitivity (95% CI: 66.4% to 100%) and 57.1% specificity (95% CI: 26.3% to 88.0%). In COVID-19 pneumonia, qualitative and quantitative analyses of lung CT images have high sensitivity but moderate to low specificity to detect histopathological fibrosis. Pseudofibrotic CT findings do not always correspond to increased collagen deposition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Pulmonar / Tomografía Computarizada por Rayos X / Colágeno / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Observational_studies / Qualitative_research / Risk_factors_studies Límite: Aged / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Pulmonar / Tomografía Computarizada por Rayos X / Colágeno / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Observational_studies / Qualitative_research / Risk_factors_studies Límite: Aged / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza