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Airway Epithelial Cells Drive Airway Smooth Muscle Cell Phenotype Switching to the Proliferative and Pro-inflammatory Phenotype.
O'Sullivan, M J; Jang, J H; Panariti, A; Bedrat, A; Ijpma, G; Lemos, B; Park, J A; Lauzon, A M; Martin, J G.
Afiliación
  • O'Sullivan MJ; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC, Canada.
  • Jang JH; T.H. Chan School of Public Health, Harvard University, Boston, MA, United States.
  • Panariti A; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC, Canada.
  • Bedrat A; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC, Canada.
  • Ijpma G; T.H. Chan School of Public Health, Harvard University, Boston, MA, United States.
  • Lemos B; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC, Canada.
  • Park JA; T.H. Chan School of Public Health, Harvard University, Boston, MA, United States.
  • Lauzon AM; T.H. Chan School of Public Health, Harvard University, Boston, MA, United States.
  • Martin JG; Meakins-Christie Laboratories, McGill University Health Centre, Montreal, QC, Canada.
Front Physiol ; 12: 687654, 2021.
Article en En | MEDLINE | ID: mdl-34295265
The increased mass of airway smooth muscle (ASM) in the airways of asthmatic patients may contribute to the pathology of this disease by increasing the capacity for airway narrowing. Evidence for the airway epithelium as a participant in ASM remodeling is accruing. To investigate mechanisms by which airway epithelial cells induce ASM cell (ASMC) proliferation, we have employed a co-culture model to explore markers of ASMC proliferative phenotype. Co-culture with epithelial cells led to incorporation of bromodeoxyuridine into ASMCs, indicating augmented proliferation and an associated increase in mRNA of the pro-proliferative co-transcription factor Elk1. Although the mitogen heparin-binding epidermal growth factor (HB-EGF) was augmented in the co-culture supernatant, the ASMC epidermal growth factor receptor (EGFR), an effector of HB-EGF induced proliferation, did not mediate epithelial-induced proliferation. The co-culture increased the expression of ASMC mRNA for the pro-inflammatory cytokines IL-6 and IL-8 as well as the pro-proliferative microRNA miR-210. The transcriptional repressor Max-binding protein (Mnt), a putative target of miR-210, was transcriptionally repressed in co-cultured ASMCs. Together, these data indicate that the airway epithelium-induced proliferative phenotype of ASMCs is not driven by EGFR signaling, but rather may be dependent on miR210 targeting of tumor suppressor Mnt.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Physiol Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Physiol Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza