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Variable response of arsenic contaminated groundwater microbial community to electron acceptor regime revealed by microcosm based high-throughput sequencing approach.
Saha, Anumeha; Mohapatra, Balaram; Kazy, Sufia Khannam; Sar, Pinaki.
Afiliación
  • Saha A; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
  • Mohapatra B; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
  • Kazy SK; Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, West Bengal, India.
  • Sar P; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
Article en En | MEDLINE | ID: mdl-34284694
Arsenic (As) mobilization in alluvial aquifers is facilitated by microbially catalyzed redox transformations that depend on the availability of electron acceptors (EAs). In this study, the response of an As-contaminated groundwater microbial community from West Bengal, India towards varied EAs was elucidated through microcosm based 16S rRNA gene amplicon sequencing. Acinetobacter, Deinococcus, Nocardioides, etc., and several unclassified bacteria (Ignavibacteria) and archaea (Bathyarchaeia, Micrarchaeia) previously not reported from As-contaminated groundwater of West Bengal, characterized the groundwater community. Distinct shifts in community composition were observed in response to various EAs. Enrichment of operational taxonomic units (OTUs) affiliated to Denitratisoma (NO3-), Spirochaetaceae (Mn4+), Deinococcus (As5+), Ruminiclostridium (Fe3+), Macellibacteroides (SO42-), Holophagae-Subgroup 7 (HCO3-), Dechloromonas and Geobacter (EA mixture) was noted. Alternatively, As3+ amendment as electron donor allowed predominance of Rhizobium. Taxonomy based functional profiling highlighted the role of chemoorganoheterotrophs capable of concurrent reduction of NO3-, Fe3+, SO42-, and As biotransformation in As-contaminated groundwater of West Bengal. Our analysis revealed two major aspects of the community, (a) taxa selective toward responding to the EAs, and (b) multifaceted nature of taxa appearing in abundance in response to multiple substrates. Thus, the results emphasized the potential of microbial community members to influence the biogeochemical cycling of As and other dominant anions/cations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arsénico / Contaminantes Químicos del Agua / Agua Subterránea / Microbiota Idioma: En Revista: J Environ Sci Health A Tox Hazard Subst Environ Eng Asunto de la revista: TOXICOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arsénico / Contaminantes Químicos del Agua / Agua Subterránea / Microbiota Idioma: En Revista: J Environ Sci Health A Tox Hazard Subst Environ Eng Asunto de la revista: TOXICOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido