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Propofol alleviates neuropathic pain in chronic constriction injury rat models via the microRNA-140-3p/Jagged-1 peptide/Notch signaling pathway.
Cheng, Fang; Qin, Wei; Yang, Ai-Xing; Yan, Feng-Feng; Chen, Yu; Ma, Jian-Xin.
Afiliación
  • Cheng F; Department of Anesthesiology and Pain Clinic, The Affiliated Lianyungang Oriental Hospital of Bengbu Medical College, Lianyungang, Jiangsu, China.
  • Qin W; Department of Critical Care Medicine, The Affiliated Lianyungang Oriental Hospital of Bengbu Medical College, Lianyungang, Jiangsu, China.
  • Yang AX; Department of Anesthesiology and Pain Clinic, The Affiliated Lianyungang Oriental Hospital of Bengbu Medical College, Lianyungang, Jiangsu, China.
  • Yan FF; Department of Anesthesiology and Pain Clinic, The Affiliated Lianyungang Oriental Hospital of Bengbu Medical College, Lianyungang, Jiangsu, China.
  • Chen Y; Department of Anesthesiology and Pain Clinic, The Affiliated Lianyungang Oriental Hospital of Bengbu Medical College, Lianyungang, Jiangsu, China.
  • Ma JX; Department of Oncology, The Affiliated Lianyungang Oriental Hospital of Bengbu Medical College, Lianyungang, Jiangsu, China.
Synapse ; 75(10): e22219, 2021 10.
Article en En | MEDLINE | ID: mdl-34269482
Chronic constriction injury (CCI) of the sciatic nerve was used to establish neuropathic pain (NP) models in rats. CCI rats were then treated with propofol (Pro) and their paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured. In addition, the expression patterns of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and IL-10 were detected. CCI rats treated with propofol were further injected with antagomiR-140-3p to verify the role of miR-140-3p in propofol's analgesic actions. In addition to confirming the relationship between miR-140-3p and JAG1, the expression patterns of JAG1 itself were detected. Propofol-treated CCI rats were also injected with Ad-JAG1 (adenovirus-packaged JAG1 overexpression vector and Ad-NC) to test the role of JAG1 in propofol's analgesic mechanism of action. Finally, the levels of JAG1 and Notch pathway-related proteins were detected RESULTS: Propofol was found to alleviate NP, including thermal hyperalgesia and mechanical pain threshold. Propofol could also ameliorate neuroinflammation by up-regulating the expression of IL-10 and inhibiting the release of TNF-α and IL-1ß. Mechanically, propofol enhanced the amount of miR-140-3p in CCI rats via the regulation of JAG1. Down-regulation of miR-140-3p, or up-regulation of JAG1, could reduce the protective effect of propofol against NP. Propofol inhibited the activation of Notch signaling via miR-140-3p/JAG1 to realize its analgesic effect CONCLUSION: Our findings indicated that propofol inhibits inflammatory responses and the Notch signaling pathway via miR-140-3p/JAG1 to alleviate NP. These data provide evidence to support a potential clinical therapy for NP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propofol / MicroARNs / Neuralgia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Synapse Asunto de la revista: NEUROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propofol / MicroARNs / Neuralgia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Synapse Asunto de la revista: NEUROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos