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Visualization of Arabidopsis root system architecture in 3D by refraction-contrast X-ray micro-computed tomography.
Kurogane, Tomofumi; Tamaoki, Daisuke; Yano, Sachiko; Tanigaki, Fumiaki; Shimazu, Toru; Kasahara, Haruo; Yamauchi, Daisuke; Uesugi, Kentaro; Hoshino, Masato; Kamisaka, Seiichiro; Mineyuki, Yoshinobu; Karahara, Ichirou.
Afiliación
  • Kurogane T; Graduate School of Science and Engineering for Education, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
  • Tamaoki D; Faculty of Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
  • Yano S; Human Spaceflight Technology Directorate, Japan Aerospace Exploration Agency, 2-1-1 Sengen, Tsukuba 305-8505, Japan.
  • Tanigaki F; Human Spaceflight Technology Directorate, Japan Aerospace Exploration Agency, 2-1-1 Sengen, Tsukuba 305-8505, Japan.
  • Shimazu T; Space Utilization Promotion Department, Japan Space Forum, 3-2-1 Kandasurugadai, Tokyo 101-0062, Japan.
  • Kasahara H; ISS Utilization and Operations Department, Japan Manned Space Systems Corporation, 1-1-26 Kawaguchi, Tsuchiura 300-0033, Japan.
  • Yamauchi D; Department of Life Science, Graduate School of Science, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
  • Uesugi K; Scattering and Imaging Division, Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
  • Hoshino M; Scattering and Imaging Division, Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
  • Kamisaka S; Faculty of Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
  • Mineyuki Y; Department of Life Science, Graduate School of Science, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
  • Karahara I; Faculty of Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
Microscopy (Oxf) ; 70(6): 536-544, 2021 Nov 24.
Article en En | MEDLINE | ID: mdl-34264299
Plant roots change their morphological traits in order to adapt themselves to different environmental conditions, resulting in the alteration of the root system architecture. To understand this mechanism, it is essential to visualize the morphology of the entire root system. To reveal effects of long-term alteration of gravity environment on root system development, we have performed an experiment in the International Space Station using Arabidopsis plants and obtained dried root systems grown in rockwool slabs. The X-ray computed tomography (CT) technique using industrial X-ray scanners has been introduced to visualize the root system architecture of crop species grown in soil in 3D non-invasively. In the case of the present study, however, the root system of Arabidopsis is composed of finer roots compared with typical crop plants and rockwool is also composed of fibers having similar dimension to that of the roots. A higher spatial resolution imaging method is required for distinguishing roots from rockwool. Therefore, in the present study, we tested refraction-contrast X-ray micro-CT using coherent X-ray optics available at the beamline of the synchrotron radiation facility SPring-8 for bio-imaging. We have found that a wide field of view but with low resolution obtained at the experimental Hutch 3 of this beamline provided an overview map of the root systems, while a narrow field of view but with high resolution obtained at the experimental Hutch 1 provided an extended architecture of the secondary roots, by a clear distinction between roots and individual rockwool fibers, resulting in the successful tracing of these roots from their basal regions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microscopy (Oxf) Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microscopy (Oxf) Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido