Immunofluorescence studies with 4-acetamido-4'-isothiocyanato stilbene-2,2'-disulphonic acid (SITS).
J Immunol Methods
; 19(2-3): 101-9, 1978.
Article
en En
| MEDLINE
| ID: mdl-342607
The application of the fluorochrome 4-acetamido-4'-isothiocyanato stilbene-2,2'-disulphonic acid (SITS) in immunofluorescence was studied. The optimal excitation wave length is 350 nm, and optimal fluorescence is obtained at 420 nm. After purification of the commercial compound, conjugation is performed in a strong buffer at pH 9.0-9.5. SITS conjugates were very satisfactory for immunofluorescence studies of the cytoplasmic antigens of cell preparations, but their blue emission was difficult to distinguish from autofluorescence in sections of human tissues. Good results with immunofluorescence on membrane bound antigens were obtained by using an ultra-violet laser beam as light source. SITS can be used simultaneously with FITC and TRITC conjugates thus making it possible to show three antigens in one preparation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estilbenos
/
Técnica del Anticuerpo Fluorescente
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1978
Tipo del documento:
Article
Pais de publicación:
Países Bajos