Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels.
Sci Rep
; 11(1): 14288, 2021 07 12.
Article
en En
| MEDLINE
| ID: mdl-34253742
Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared several parameters including oocyte source, donor cell and breed differences, transfer methods, embryo formation and pregnancy rates and maintenance following SCNT. We successfully achieved 47 pregnancies, 28 births and 19 cloned offspring who are at present healthy and have developed normally. Here we report cloned camels from surgical embryo transfer and correlate blastocyst formation rates with the ability to achieve pregnancies. We found no difference in the parameters affecting production of clones by camel breed, and show clear differences on oocyte source in cloning outcomes. Taken together we demonstrate that large scale cloning of camels is possible and that further improvements can be achieved.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Blastocisto
/
Camelus
/
Ultrasonografía
/
Técnicas de Cultivo de Embriones
/
Transferencia de Embrión
/
Técnicas de Transferencia Nuclear
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Sci Rep
Año:
2021
Tipo del documento:
Article
País de afiliación:
Emiratos Árabes Unidos
Pais de publicación:
Reino Unido