Your browser doesn't support javascript.
loading
Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses.
Ravindran, Resmi; McReynolds, Cindy; Yang, Jun; Hammock, Bruce D; Ikram, Aamer; Ali, Amna; Bashir, Adnan; Zohra, Tanzeel; Chang, W L William; Hartigan-O'Connor, Dennis J; Rashidi, Hooman H; Khan, Imran H.
Afiliación
  • Ravindran R; Department of Pathology and Laboratory Medicine, University of California, Davis, CA, United States of America.
  • McReynolds C; Department of Entomology and Nematology, University of California, Davis, CA, United States of America.
  • Yang J; Department of Entomology and Nematology, University of California, Davis, CA, United States of America.
  • Hammock BD; Department of Entomology and Nematology, University of California, Davis, CA, United States of America.
  • Ikram A; National Institutes of Health, Rawalpindi, Pakistan.
  • Ali A; National Institutes of Health, Rawalpindi, Pakistan.
  • Bashir A; National Institutes of Health, Rawalpindi, Pakistan.
  • Zohra T; National Institutes of Health, Rawalpindi, Pakistan.
  • Chang WLW; California National Primate Research Center, University of California, Davis, CA, United States of America.
  • Hartigan-O'Connor DJ; Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, United States of America.
  • Rashidi HH; California National Primate Research Center, University of California, Davis, CA, United States of America.
  • Khan IH; Department of Pathology and Laboratory Medicine, University of California, Davis, CA, United States of America.
PLoS One ; 16(7): e0254367, 2021.
Article en En | MEDLINE | ID: mdl-34242356
COVID-19 serological test must have high sensitivity as well as specificity to rule out cross-reactivity with common coronaviruses (HCoVs). We have developed a quantitative multiplex test, measuring antibodies against spike (S) proteins of SARS-CoV-2, SARS-CoV, MERS-CoV, and common human coronavirus strains (229E, NL63, OC43, HKU1), and nucleocapsid (N) protein of SARS-CoV viruses. Receptor binding domain of S protein of SARS-CoV-2 (S-RBD), and N protein, demonstrated sensitivity (94% and 92.5%, respectively) in COVID-19 patients (n = 53), with 98% specificity in non-COVID-19 respiratory-disease (n = 98), and healthy-controls (n = 129). Anti S-RBD and N antibodies appeared five to ten days post-onset of symptoms, peaking at approximately four weeks. The appearance of IgG and IgM coincided while IgG subtypes, IgG1 and IgG3 appeared soon after the total IgG; IgG2 and IgG4 remained undetectable. Several inflammatory cytokines/chemokines were found to be elevated in many COVID-19 patients (e.g., Eotaxin, Gro-α, CXCL-10 (IP-10), RANTES (CCL5), IL-2Rα, MCP-1, and SCGF-b); CXCL-10 was elevated in all. In contrast to antibody titers, levels of CXCL-10 decreased with the improvement in patient health suggesting it as a candidate for disease resolution. Importantly, anti-N antibodies appear before S-RBD and differentiate between vaccinated and infected people-current vaccines (and several in the pipeline) are S protein-based.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina G / Inmunoglobulina M / Quimiocinas / Glicoproteína de la Espiga del Coronavirus / Proteínas de la Nucleocápside de Coronavirus / SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina G / Inmunoglobulina M / Quimiocinas / Glicoproteína de la Espiga del Coronavirus / Proteínas de la Nucleocápside de Coronavirus / SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos