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Effects of cigarette smoke extract on bronchial epithelial cells stimulated with Cryptococcus neoformans.
Pereira, Aline Beatriz Mahler; Oliveira, Jhony Robison; Souza, Ana Leticia Julio; Andrade-Silva, Leonardo; Silva, Marcos Vinicius; Silva, Paulo Roberto; Silva-Vergara, Mario Leon; Rogerio, Alexandre Paula.
Afiliación
  • Pereira ABM; Laboratory of Experimental Immunopharmacology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Street Vigário Carlos 162, Uberaba, MG, 38025-380, Brazil.
  • Oliveira JR; Laboratory of Experimental Immunopharmacology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Street Vigário Carlos 162, Uberaba, MG, 38025-380, Brazil.
  • Souza ALJ; Laboratory of Experimental Immunopharmacology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Street Vigário Carlos 162, Uberaba, MG, 38025-380, Brazil.
  • Andrade-Silva L; Laboratory of Mycology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
  • Silva MV; Laboratory of Immunology, Institute Department of Clinical Medicine, of Health Sciences, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
  • Silva PR; Laboratory of Experimental Immunopharmacology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Street Vigário Carlos 162, Uberaba, MG, 38025-380, Brazil.
  • Silva-Vergara ML; Laboratory of Mycology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
  • Rogerio AP; Laboratory of Experimental Immunopharmacology, Department of Clinical Medicine, Institute of Health Sciences, Federal University of Triangulo Mineiro, Street Vigário Carlos 162, Uberaba, MG, 38025-380, Brazil. alexandre.rogerio@uftm.edu.br.
Med Microbiol Immunol ; 210(4): 221-233, 2021 Aug.
Article en En | MEDLINE | ID: mdl-34228244
In the airways, the adhesion of Cryptococcus neoformans with airway epithelial cells is crucial for the establishment of cryptococcosis. Tobacco smoke is considered a risk factor for cryptococcosis. Here, we evaluated the effects of cigarette smoke extract (CSE) on human bronchial epithelial cells (BEAS-2B) stimulated with C. neoformans. Multiplicities of infection (MOIs) of 1-100 of C. neoformans per cell led to increased IL-8 production and no cytotoxic effects when compared to those of controls. C. neoformans (MOI 100) also significantly increased the concentration of IL-6. In cells stimulated with CSE doses (1.0, 2.5 and 5.0%) from one or five cigarettes, increased IL-1ß production was observed only in doses from one (1.0%) and five (2.5%) cigarettes when compared to that of controls. However, only 1.0% CSE failed to show cytotoxic effects. In addition, CSE significantly increased the concentration of IL-8. Cells stimulated with both CSE and C. neoformans demonstrated a reduction in IL-6/STAT3 signalling compared to that in cells stimulated by C. neoformans. In addition, a significant increase in IL-10 production was also observed. No alterations in NF-kB or ICAM-1 expression were observed among the groups. The combination of CSE and C. neoformans favoured the increase of fungal numbers and extracellular adhering of C. neoformans on BEAS-2B cells. In addition, the internalization of C. neoformans on BEAS-2B cells was reduced after CSE stimulation. In conclusion, the association of CSE and C. neoformans induced an anti-inflammatory effect in bronchial epithelial cells, which might favour the development of C. neoformans infection in the airways.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Humo / Citocinas / Criptococosis / Cryptococcus neoformans / Células Epiteliales / Productos de Tabaco Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Med Microbiol Immunol Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Humo / Citocinas / Criptococosis / Cryptococcus neoformans / Células Epiteliales / Productos de Tabaco Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Med Microbiol Immunol Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania