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Intra-abdominal EWSR1/FUS-CREM-rearranged malignant epithelioid neoplasms: two cases of an emerging aggressive entity with emphasis on misleading immunophenotype.
Agaimy, Abbas; Stoehr, Robert; Otto, Mike; Bräsen, Jan Hinrich; Pfarr, Nicole; Konukiewitz, Björn; Kasajima, Atsuko; Hartmann, Arndt; Klöppel, Günter.
Afiliación
  • Agaimy A; Institute of Pathology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University Hospital Erlangen (UKER), Erlangen, Germany. abbas.agaimy@uk-erlangen.de.
  • Stoehr R; Comprehensive Cancer Center Erlangen-EMN (CCC ER-EMN), Erlangen, Germany. abbas.agaimy@uk-erlangen.de.
  • Otto M; Institute of Pathology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University Hospital Erlangen (UKER), Erlangen, Germany.
  • Bräsen JH; Comprehensive Cancer Center Erlangen-EMN (CCC ER-EMN), Erlangen, Germany.
  • Pfarr N; Medical Center of Histology, Cytology and Molecular Diagnostics Trier LLC (GmbH), Trier, Germany.
  • Konukiewitz B; Institute of Pathology, Hannover Medical School, Hannover, Germany.
  • Kasajima A; Institute of Pathology, Technical University Munich, Munich, Germany.
  • Hartmann A; Institute of Pathology, Technical University Munich, Munich, Germany.
  • Klöppel G; Institute of Pathology, Technical University Munich, Munich, Germany.
Virchows Arch ; 480(2): 481-486, 2022 Feb.
Article en En | MEDLINE | ID: mdl-34228212
CREB family (CREB1, ATF1, and CREM) gene fusions are defining markers in diverse mesenchymal neoplasms (clear cell sarcoma, angiomatoid fibrous histiocytoma, and others). However, neoplasms harboring EWSR1-CREM/FUS-CREM fusions are rare and poorly characterized. We describe two cases (55-year-old male with 7.5 cm renal mass and 32-year-old female with 5.5 cm mesenteric mass) illustrating their misleading immunophenotypes. Histologically, both showed eosinophilic and focally clear epithelioid cells arranged into sheets, nests, and trabeculae. Immunohistochemistry showed ALK, EMA, and AE1/AE3 immunoreactivity suggesting ALK-rearranged renal cell carcinoma (Case 1) and coexpression of keratin, EMA, synaptophysin, and chromogranin-A, suggesting neuroendocrine neoplasm (Case 2). Targeted RNA sequencing revealed EWSR1-CREM (Case 1) and FUS-CREM (Case 2) fusions. These cases add to the spectrum of CREM fusion-positive intra-abdominal epithelioid neoplasms. Their unusual immunophenotype and unexpected sites represent major pitfalls, underline a wide differential diagnosis, and emphasize the value of molecular testing in correctly diagnosing them.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Fusión Oncogénica / Histiocitoma Fibroso Maligno Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Virchows Arch Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Fusión Oncogénica / Histiocitoma Fibroso Maligno Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Virchows Arch Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania