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Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes.
Nong, Daguan; Haviland, Zachary K; Kuntz, Kate Vasquez; Tien, Ming; Anderson, Charles T; Hancock, William O.
Afiliación
  • Nong D; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
  • Haviland ZK; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
  • Kuntz KV; Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
  • Tien M; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.
  • Anderson CT; Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
  • Hancock WO; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Biomed Opt Express ; 12(6): 3253-3264, 2021 Jun 01.
Article en En | MEDLINE | ID: mdl-34221658
We describe a multimodal microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines interference reflection microscopy (IRM) with multi-wavelength total internal reflectance fluorescence microscopy (TIRFM). The microscope can localize quantum dots with a precision of 2.8 nm at 100 frames/s, and was used to image the dynamics of the cellulase, Cel7a interacting with surface-immobilized cellulose. The instrument, which was built with off-the-shelf components and is controlled by custom software, is suitable for tracking other degradative enzymes such as collagenases, as well as motor proteins moving along immobilized tracks.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos