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Seed specifically over-expressing DGAT2A enhances oil and linoleic acid contents in soybean seeds.
Jing, Guangqin; Tang, Daoping; Yao, Yao; Su, Youke; Shen, Yue; Bai, Yang; Jing, Wen; Zhang, Qun; Lin, Feng; Guo, Dongquan; Zhang, Wenhua.
Afiliación
  • Jing G; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
  • Tang D; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
  • Yao Y; Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, PR China.
  • Su Y; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
  • Shen Y; Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, PR China.
  • Bai Y; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
  • Jing W; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
  • Zhang Q; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
  • Lin F; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: fenglin123@njau.edu.cn.
  • Guo D; Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, PR China. Electronic address: xzgdq@126.com.
  • Zhang W; College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
Biochem Biophys Res Commun ; 568: 143-150, 2021 09 03.
Article en En | MEDLINE | ID: mdl-34217012
Triacylglycerol (TAG), a main component of oil, is mainly biosynthesized by diacylglycerol acyltransferase (DGAT), which is critical for oil accumulation in plants. Intensive focus has been on DGAT2 functioning in unsaturated fatty acids biosynthesis. In this study, we analyzed the coding sequence (CDS) and amino acid sequence of GmDGAT2A and determined its key active sites through site-directed mutagenesis. As a consequence, H132, G201, and P152-X-I154-K155 were found to be essential active sites for GmDGAT2A. The spatial structure of the protein may bring the three active sites into close proximity, constituting an active domain. Additionally, N-terminus of GmDGAT2A was found to be an important regulator for the activity. Further, in vitro activity results uncovered GmDGAT2A was prone to utilize C18:2-CoA as the substrate. Consequently, overexpression of GmDGAT2A driven by a seed-specific promoter of Gmole1 in soybean significantly increased linoleic acid content specifically and total oil content, concomitant with accelerated elongation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Semillas / Glycine max / Ácido Linoleico / Diacilglicerol O-Acetiltransferasa Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Semillas / Glycine max / Ácido Linoleico / Diacilglicerol O-Acetiltransferasa Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos