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Integrated Insight into the Molecular Mechanisms of Spontaneous Abortion during Early Pregnancy in Pigs.
Zang, Xupeng; Gu, Ting; Wang, Wenjing; Zhou, Chen; Ding, Yue; Gu, Shengchen; Xu, Zhiqian; Xie, Yanshe; Li, Zicong; Cai, Gengyuan; Hu, Bin; Hong, Linjun; Wu, Zhenfang.
Afiliación
  • Zang X; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Gu T; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Wang W; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
  • Zhou C; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Ding Y; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Gu S; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
  • Xu Z; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Xie Y; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Li Z; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
  • Cai G; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Hu B; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
  • Hong L; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
  • Wu Z; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci ; 22(12)2021 Jun 21.
Article en En | MEDLINE | ID: mdl-34205766
Due to the high rate of spontaneous abortion (SAB) in porcine pregnancy, there is a major interest and concern on commercial pig farming worldwide. Whereas the perturbed immune response at the maternal-fetal interface is an important mechanism associated with the spontaneous embryo loss in the early stages of implantation in porcine, data on the specific regulatory mechanism of the SAB at the end stage of the implantation remains scant. Therefore, we used high-throughput sequencing and bioinformatics tools to analyze the healthy and arresting endometrium on day 28 of pregnancy. We identified 639 differentially expressed lncRNAs (DELs) and 2357 differentially expressed genes (DEGs) at the end stage of implantation, and qRT-PCR was used to verify the sequencing data. Gene set variation analysis (GSVA), gene set enrichment analysis (GSEA), and immunohistochemistry analysis demonstrated weaker immune response activities in the arresting endometrium compared to the healthy one. Using the lasso regression analysis, we screened the DELs and constructed an immunological competitive endogenous RNA (ceRNA) network related to SAB, including 4 lncRNAs, 11 miRNAs, and 13 genes. In addition, Blast analysis showed the applicability of the constructed ceRNA network in different species, and subsequently determined HOXA-AS2 in pigs. Our study, for the first time, demonstrated that the SAB events at the end stages of implantation is associated with the regulation of immunobiological processes, and a specific molecular regulatory network was obtained. These novel findings may provide new insight into the possibility of increasing the litter size of sows, making pig breeding better and thus improving the efficiency of animal husbandry production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Porcinos / Aborto Espontáneo / ARN Largo no Codificante Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Porcinos / Aborto Espontáneo / ARN Largo no Codificante Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza