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NQS-Doped PDMS Solid Sensor: From Water Matrix to Urine Enzymatic Application.
Martínez-Aviñó, Adrià; Hakobyan, Lusine; Ballester-Caudet, Ana; Moliner-Martínez, Yolanda; Molins-Legua, Carmen; Campíns-Falcó, Pilar.
Afiliación
  • Martínez-Aviñó A; MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
  • Hakobyan L; MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
  • Ballester-Caudet A; MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
  • Moliner-Martínez Y; MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
  • Molins-Legua C; MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
  • Campíns-Falcó P; MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
Biosensors (Basel) ; 11(6)2021 Jun 08.
Article en En | MEDLINE | ID: mdl-34201174
The development of in situ analytical devices has gained outstanding scientific interest. A solid sensing membrane composed of 1,2-naphthoquinone-4-sulfonate (NQS) derivatizing reagent embedded into a polymeric polydimethylsiloxane (PDMS) composite was proposed for in situ ammonium (NH4+) and urea (NH2CONH2) analysis in water and urine samples, respectively. Satisfactory strategies were also applied for urease-catalyzed hydrolysis of urea, either in solution or glass-supported urease immobilization. Using diffuse reflectance measurements combined with digital image processing of color intensity (RGB coordinates), qualitative and quantitative analyte detection was assessed after the colorimetric reaction took place inside the sensing membrane. A suitable linear relationship was found between the sensor response and analyte concentration, and the results were validated by a thymol-PDMS-based sensor based on the Berthelot reaction. The suggested sensing device offers advantages such as rapidity, versatility, portability, and employment of non-toxic reagents that facilitate in situ analysis in an energy-efficient manner.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Urea / Ureasa / Naftoquinonas Tipo de estudio: Qualitative_research Idioma: En Revista: Biosensors (Basel) Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Urea / Ureasa / Naftoquinonas Tipo de estudio: Qualitative_research Idioma: En Revista: Biosensors (Basel) Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza