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In Vivo Assessment of Hypoxia Levels in Pancreatic Tumors Using a Dual-Modality Ultrasound/Photoacoustic Imaging System.
Wang, Yuhling; Jhang, De-Fu; Tsai, Chia-Hua; Chiang, Nai-Jung; Tsao, Chia-Hui; Chuang, Chiung-Cheng; Chen, Li-Tzong; Chang, Wun-Shaing Wayne; Liao, Lun-De.
Afiliación
  • Wang Y; Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, No. 35, Keyan Rd., Zhunan Township, Miaoli 35053, Taiwan.
  • Jhang DF; Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, No. 35, Keyan Rd., Zhunan Township, Miaoli 35053, Taiwan.
  • Tsai CH; Department of Biomedical Engineering, College of Engineering, Chung Yuan Christian University, No. 200, Chung Pei Road, Chung Li District, Taoyuan 32023, Taiwan.
  • Chiang NJ; Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, No. 35, Keyan Rd., Zhunan Township, Miaoli 35053, Taiwan.
  • Tsao CH; National Institute of Cancer Research, National Health Research Institutes, No. 35, Keyan Rd., Zhunan Township, Miaoli 35053, Taiwan.
  • Chuang CC; Department of Oncology, National Cheng Kung University Hospital, Tainan 70403, Taiwan.
  • Chen LT; Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, No. 35, Keyan Rd., Zhunan Township, Miaoli 35053, Taiwan.
  • Chang WW; Department of Biomedical Engineering, College of Engineering, Chung Yuan Christian University, No. 200, Chung Pei Road, Chung Li District, Taoyuan 32023, Taiwan.
  • Liao LD; National Institute of Cancer Research, National Health Research Institutes, No. 35, Keyan Rd., Zhunan Township, Miaoli 35053, Taiwan.
Micromachines (Basel) ; 12(6)2021 Jun 07.
Article en En | MEDLINE | ID: mdl-34200388
Noninvasive anatomical and functional imaging has become an essential tool to evaluate tissue oxygen saturation dynamics in preclinical or clinical studies of hypoxia. Our dual-wavelength technique for photoacoustic (PA) imaging based on the differential absorbance spectrum of oxyhemoglobin (oxy-Hb) and deoxyhemoglobin (deoxy-Hb) can quantify tissue oxygen saturation using the intrinsic contrast property. PA imaging of tissue oxygen saturation can be used to monitor tumor-related hypoxia, which is a particularly relevant functional parameter of the tumor microenvironment that has a strong influence on tumor aggressiveness. The simultaneous acquisition of anatomical and functional information using dual-modality ultrasound (US) and PA imaging technology enhances the preclinical applicability of the method. Here, the developed dual-modality US/PA system was used to measure relative tissue oxygenation using the dual-wavelength technique. Tissue oxygen saturation was quantified in a pancreatic tumor mouse model. The differences in tissue oxygenation were detected by comparing pancreatic samples from normal and tumor-bearing mice at various time points after implantation. The use of an in vivo pancreatic tumor model revealed changes in hypoxia at various stages of tumor growth. The US/PA imaging data positively correlated with the results of immunohistochemical staining for hypoxia. Thus, our dual-modality US/PA imaging system can be used to reliably assess and monitor hypoxia in pancreatic tumor mouse models. These findings enable the use of a combination of US and PA imaging to acquire anatomical and functional information on tumor growth and to evaluate treatment responses in longitudinal preclinical studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Micromachines (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Micromachines (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza