Your browser doesn't support javascript.
loading
Supplementation with asiatic acid during in vitro maturation improves porcine oocyte developmental competence by regulating oxidative stress.
Qi, Jia-Jia; Li, Xiao-Xia; Zhang, Yan; Diao, Yun-Fei; Hu, Wei-Yi; Wang, Da-Li; Jiang, Hao; Zhang, Jia-Bao; Sun, Bo-Xing; Liang, Shuang.
Afiliación
  • Qi JJ; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.
  • Li XX; College of Animal Science and Technology, Jilin Agriculture Science and Technology University, Jilin, China; Jilin Province Key Laboratory of Preventive Veterinary Medicine, Jilin Agriculture Science and Technology University, Jilin, China.
  • Zhang Y; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China; Department of Animal Science, Chungbuk National University, Cheongju, South Korea.
  • Diao YF; College of Animal Science and Technology, Jilin Agriculture Science and Technology University, Jilin, China; Jilin Province Key Laboratory of Preventive Veterinary Medicine, Jilin Agriculture Science and Technology University, Jilin, China.
  • Hu WY; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.
  • Wang DL; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.
  • Jiang H; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.
  • Zhang JB; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.
  • Sun BX; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China. Electronic address: Sunpathing@vip.163.com.
  • Liang S; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China. Electronic address: liangshuang85@jlu.edu.cn.
Theriogenology ; 172: 169-177, 2021 Sep 15.
Article en En | MEDLINE | ID: mdl-34174755
Asiatic acid is a natural triterpene found in Centella asiatica that acts as an effective free radical scavenger. Our previous research showed that asiatic acid delayed porcine oocyte ageing in vitro and improved preimplantation embryo development competence in vitro; however, the protective effects of asiatic acid against oxidative stress in porcine oocyte maturation are still unclear. Here, we investigated the effects of asiatic acid on porcine oocyte in vitro maturation (IVM) and subsequent embryonic development competence after parthenogenetic activation (PA) and in vitro fertilization (IVF). The results of the present research showed that 10 µM asiatic acid supplementation did not affect the expansion of cumulus cells or polar body extrusion of porcine oocytes, while asiatic acid application significantly increased the subsequent blastocyst formation rate and quality of porcine PA and IVF embryos. Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that induces oxidative stress in porcine oocytes. As expected, asiatic acid supplementation not only decreased intracellular ROS levels but also attenuated H2O2-induced intracellular ROS generation. Further analysis revealed that asiatic acid supplementation enhanced intracellular glutathione production, mitochondrial membrane potential, and ATP generation at the end of IVM. In summary, our results reveal that asiatic acid supplementation exerts beneficial effects on porcine oocytes by regulating oxidative stress during the IVM process and could act as a potential antioxidant in porcine oocytes matured in vitro production systems.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Maduración In Vitro de los Oocitos / Peróxido de Hidrógeno Límite: Animals Idioma: En Revista: Theriogenology Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Maduración In Vitro de los Oocitos / Peróxido de Hidrógeno Límite: Animals Idioma: En Revista: Theriogenology Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos