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A generator of peroxynitrite activatable with red light.
Parisi, Cristina; Failla, Mariacristina; Fraix, Aurore; Menilli, Luca; Moret, Francesca; Reddi, Elena; Rolando, Barbara; Spyrakis, Francesca; Lazzarato, Loretta; Fruttero, Roberta; Gasco, Alberto; Sortino, Salvatore.
Afiliación
  • Parisi C; PhotoChemLab, Department of Drug and Health Sciences, University of Catania I-95125 Italy ssortino@unict.it.
  • Failla M; Department of Science and Drug Technology, University of Torino I-10125 Torino Italy Loretta.lazzarato@unito.it.
  • Fraix A; PhotoChemLab, Department of Drug and Health Sciences, University of Catania I-95125 Italy ssortino@unict.it.
  • Menilli L; Department of Biology, University of Padova I-35131 Padova Italy.
  • Moret F; Department of Biology, University of Padova I-35131 Padova Italy.
  • Reddi E; Department of Biology, University of Padova I-35131 Padova Italy.
  • Rolando B; Department of Science and Drug Technology, University of Torino I-10125 Torino Italy Loretta.lazzarato@unito.it.
  • Spyrakis F; Department of Science and Drug Technology, University of Torino I-10125 Torino Italy Loretta.lazzarato@unito.it.
  • Lazzarato L; Department of Science and Drug Technology, University of Torino I-10125 Torino Italy Loretta.lazzarato@unito.it.
  • Fruttero R; Department of Science and Drug Technology, University of Torino I-10125 Torino Italy Loretta.lazzarato@unito.it.
  • Gasco A; Department of Science and Drug Technology, University of Torino I-10125 Torino Italy Loretta.lazzarato@unito.it.
  • Sortino S; PhotoChemLab, Department of Drug and Health Sciences, University of Catania I-95125 Italy ssortino@unict.it.
Chem Sci ; 12(13): 4740-4746, 2021 Mar 04.
Article en En | MEDLINE | ID: mdl-34163730
The generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) as "unconventional" therapeutics with precise spatiotemporal control by using light stimuli may open entirely new horizons for innovative therapeutic modalities. Among ROS and RNS, peroxynitrite (ONOO-) plays a dominant role in chemistry and biology in view of its potent oxidizing power and cytotoxic action. We have designed and synthesized a molecular hybrid based on benzophenothiazine as a red light-harvesting antenna joined to an N-nitroso appendage through a flexible spacer. Single photon red light excitation of this molecular construct triggers the release of nitric oxide (˙NO) and simultaneously produces superoxide anions (O2˙-). The diffusion-controlled reaction between these two radical species generates ONOO-, as confirmed by the use of fluorescein-boronate as a highly selective chemical probe. Besides, the red fluorescence of the hybrid allows its tracking in different types of cancer cells where it is well-tolerated in the dark but induces remarkable cell mortality under irradiation with red light in a very low concentration range, with very low light doses (ca. 1 J cm-2). This ONOO- generator activatable by highly biocompatible and tissue penetrating single photon red light can open up intriguing prospects in biomedical research, where precise and spatiotemporally controlled concentrations of ONOO- are required.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido