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Gold based nano-photonic approach for point-of-care detection of circulating long non-coding RNAs.
Shandilya, Ruchita; Kumari, Roshani; Singh, Radha Dutt; Chouksey, Anju; Bhargava, Arpit; Goryacheva, Irina Yu; Mishra, Pradyumna Kumar.
Afiliación
  • Shandilya R; Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India.
  • Kumari R; Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India.
  • Singh RD; Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India.
  • Chouksey A; Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India.
  • Bhargava A; Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India.
  • Goryacheva IY; Department of General and Inorganic Chemistry, Saratov State University, Saratov, Russia.
  • Mishra PK; Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India. Electronic address: pkm_8bh@yahoo.co.uk.
Nanomedicine ; 36: 102413, 2021 08.
Article en En | MEDLINE | ID: mdl-34147663
Development of a rapid, sensitive and easy to use point of care assay for detection of circulating long non-coding RNAs (lncRNAs) is of great importance. These biomolecules possess the ability to regulate vital cellular processes and act as biomarkers for various human non-communicable diseases. The present work aimed to develop a simplified and reliable cytometric fluorescence-based approach for precise recognition of circulating lncRNAs in a given sample using biotinylated uracil-modified oligonucleotide tethered AlexaFluor488-labeled streptavidin gold colloidal (BiO-StrAG) nano-conjugates. The fluorophores in close proximity to the gold nanoparticles result in quenching of fluorescence; however, specific recognition of target lncRNAs increases this distance which causes plasmonic enhancement of fluorescence. As per the flow cytometry and fluorometry investigations, the developed methodology provides a precise and sensitive approach for detection of the target lncRNAs (up to 5 nM in any given sample). With advantages of high selectivity and feasibility, our strategy offers great potential of being developed as a promising tool for interrogating aberrant regulation of lncRNAs functions, especially indicated in various diseased states.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas de Atención de Punto / Nanopartículas del Metal / ARN Largo no Codificante / Citometría de Flujo / Oro Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas de Atención de Punto / Nanopartículas del Metal / ARN Largo no Codificante / Citometría de Flujo / Oro Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos