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Discovery of M3 Antagonist-PDE4 Inhibitor Dual Pharmacology Molecules for the Treatment of Chronic Obstructive Pulmonary Disease.
Armani, Elisabetta; Rizzi, Andrea; Capaldi, Carmelida; De Fanti, Renato; Delcanale, Maurizio; Villetti, Gino; Marchini, Gessica; Pisano, Anna Rita; Pitozzi, Vanessa; Pittelli, Maria Gloria; Trevisani, Marcello; Salvadori, Michela; Cenacchi, Valentina; Puccini, Paola; Amadei, Francesco; Pappani, Alice; Civelli, Maurizio; Patacchini, Riccardo; Baker-Glenn, Charles A G; Van de Poël, Hervé; Blackaby, Wesley P; Nash, Kevin; Amari, Gabriele.
Afiliación
  • Armani E; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Rizzi A; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Capaldi C; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • De Fanti R; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Delcanale M; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Villetti G; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Marchini G; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Pisano AR; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Pitozzi V; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Pittelli MG; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Trevisani M; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Salvadori M; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Cenacchi V; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Puccini P; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Amadei F; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Pappani A; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Civelli M; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Patacchini R; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
  • Baker-Glenn CAG; Chesterford Research Park, Charles River Discovery Research Services UK Ltd, Saffron Walden CB10 1XL, United Kingdom.
  • Van de Poël H; Chesterford Research Park, Charles River Discovery Research Services UK Ltd, Saffron Walden CB10 1XL, United Kingdom.
  • Blackaby WP; Chesterford Research Park, Charles River Discovery Research Services UK Ltd, Saffron Walden CB10 1XL, United Kingdom.
  • Nash K; Chesterford Research Park, Charles River Discovery Research Services UK Ltd, Saffron Walden CB10 1XL, United Kingdom.
  • Amari G; Nuovo Centro Ricerche, Chiesi Farmaceutici S.p.A., Largo Belloli 11/a, 43122 Parma, Italy.
J Med Chem ; 64(13): 9100-9119, 2021 07 08.
Article en En | MEDLINE | ID: mdl-34142835
In this paper, we report the discovery of dual M3 antagonist-PDE4 inhibitor (MAPI) compounds for the inhaled treatment of pulmonary diseases. The identification of dual compounds was enabled by the intuition that the fusion of a PDE4 scaffold derived from our CHF-6001 series with a muscarinic scaffold through a common linking ring could generate compounds active versus both the transmembrane M3 receptor and the intracellular PDE4 enzyme. Two chemical series characterized by two different muscarinic scaffolds were investigated. SAR optimization was aimed at obtaining M3 nanomolar affinity coupled with nanomolar PDE4 inhibition, which translated into anti-bronchospastic efficacy ex vivo (inhibition of rat trachea contraction) and into anti-inflammatory efficacy in vitro (inhibition of TNFα release). Among the best compounds, compound 92a achieved the goal of demonstrating in vivo efficacy and duration of action in both the bronchoconstriction and inflammation assays in rat after intratracheal administration.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad Pulmonar Obstructiva Crónica / Receptor Muscarínico M3 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4 / Descubrimiento de Drogas / Inhibidores de Fosfodiesterasa 4 Límite: Animals Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad Pulmonar Obstructiva Crónica / Receptor Muscarínico M3 / Fosfodiesterasas de Nucleótidos Cíclicos Tipo 4 / Descubrimiento de Drogas / Inhibidores de Fosfodiesterasa 4 Límite: Animals Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos