Your browser doesn't support javascript.
loading
Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection.
Wang, Jiarui; Han, Mengting; Roy, Anish R; Wang, Haifeng; Möckl, Leonhard; Zeng, Leiping; Moerner, W E; Qi, Lei S.
Afiliación
  • Wang J; Department of Chemistry, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
  • Han M; Department of Developmental Biology, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
  • Roy AR; Departments of Bioengineering, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
  • Wang H; Department of Chemistry, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
  • Möckl L; Departments of Bioengineering, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
  • Zeng L; Department of Chemistry, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
  • Moerner WE; Present address: Max Planck Institute for the Science of Light, Erlangen, Germany.
  • Qi LS; Departments of Bioengineering, Chemical and Systems Biology, and ChEM-H Stanford University, Stanford, California, 94305 U.S.A.
bioRxiv ; 2022 Jan 12.
Article en En | MEDLINE | ID: mdl-34127974
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects.
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos