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Evaluation of Fibroblast Viability Seeded on Acellular Human Amniotic Membrane.
Moravvej, Hamideh; Memariani, Hamed; Memariani, Mojtaba; Kabir-Salmani, Maryam; Shoae-Hassani, Alireza; Abdollahimajd, Fahimeh.
Afiliación
  • Moravvej H; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Memariani H; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Memariani M; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Kabir-Salmani M; Biomaterials and Tissue Engineering Department, Stem Cell Division, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
  • Shoae-Hassani A; Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.
  • Abdollahimajd F; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Biomed Res Int ; 2021: 5597758, 2021.
Article en En | MEDLINE | ID: mdl-34124249
BACKGROUND: Investigating the viability and proliferative rates of fibroblast cells on human amniotic membrane (HAM) as a scaffold will be an important subject for further research. The aim of this study was to assess the fibroblast viability seeded on acellular HAM, since foreskin neonatal allogenic fibroblasts seeded on HAM accelerate the wound healing process. METHODS: Fibroblasts were retrieved from the foreskin of a genetically healthy male infant, and we exploited AM of healthy term neonates to prepare the amniotic scaffold for fibroblast transfer. After cell culture, preparation of acellular HAM, and seeding of cells on HAM based on the protocol, different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI), and propidium iodide (PI) staining were employed for assessment of fibroblast viability on HAM. RESULTS: Based on the results obtained from the DAPI and PI staining, the percentage of viable cells in the former staining was clearly higher than that of the dead cells in the latter one. The results of DAPI and PI staining were in accordance with the findings of MTT assay, confirming that fibroblasts were viable and even proliferate on HAM. CONCLUSION: Our findings showed the viability of fibroblasts seeded on the acellular HAM using MTT assay, DAPI, and PI staining; however, this study had some limitations. It would be an interesting subject for future research to compare the viability and proliferation rate of fibroblasts seeded on both cellular and acellular HAM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Andamios del Tejido / Fibroblastos / Amnios Límite: Humans / Male Idioma: En Revista: Biomed Res Int Año: 2021 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Andamios del Tejido / Fibroblastos / Amnios Límite: Humans / Male Idioma: En Revista: Biomed Res Int Año: 2021 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos