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Genistein inhibits chondrogenic differentiation and mineralization of ATDC5 cells.
Kitagawa, Aya; Yamaguchi, Mana; Fujiwara, Aki; Shimizu, Muneshige; Takahashi, Azusa; Sone, Hideyuki; Kamiyama, Shin.
Afiliación
  • Kitagawa A; Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, Niigata, 950-8680, Japan.
  • Yamaguchi M; Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, Niigata, 950-8680, Japan.
  • Fujiwara A; Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, Niigata, 950-8680, Japan.
  • Shimizu M; Department of Fisheries, Faculty of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka, 424-8610, Japan.
  • Takahashi A; Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, Niigata, 950-8680, Japan.
  • Sone H; Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, Niigata, 950-8680, Japan.
  • Kamiyama S; Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, Niigata, 950-8680, Japan. Electronic address: kammy@unii.ac.jp.
Biochem Biophys Res Commun ; 566: 123-128, 2021 08 20.
Article en En | MEDLINE | ID: mdl-34119824
Isoflavones are phytoestrogens abundant in leguminous crops and are used to prevent a variety of hormonal disorders. In the present study, the effects of genistein and daidzein on the chondrogenic differentiation of ATDC5 cells were investigated. Genistein (10 µM) treatment markedly reduced production of sulfated proteoglycans and collagen fibers in the ATDC5 cells. Genistein suppressed the expression of genes involved in chondrocyte differentiation such as Sox9, Col2a1, Col10a1, Acan, and Tgfb1. Additionally, genistein significantly decreased calcium deposition in ATDC5 cells during chondrogenic differentiation; however, it increased calcification under non-chondrogenic mineralizing conditions. Daidzein exhibited a similar effect of suppressing chondrogenesis in ATDC5 cells, although its efficacy was 10-times lower than that of genistein. These findings suggest that a high concentration of genistein inhibits chondrogenesis and chondrogenic mineralization, whereas it enhances non-chondrogenic mineralization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Genisteína / Condrogénesis Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Genisteína / Condrogénesis Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos