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Hypertrophic cardiomyopathy ß-cardiac myosin mutation (P710R) leads to hypercontractility by disrupting super relaxed state.
Vander Roest, Alison Schroer; Liu, Chao; Morck, Makenna M; Kooiker, Kristina Bezold; Jung, Gwanghyun; Song, Dan; Dawood, Aminah; Jhingran, Arnav; Pardon, Gaspard; Ranjbarvaziri, Sara; Fajardo, Giovanni; Zhao, Mingming; Campbell, Kenneth S; Pruitt, Beth L; Spudich, James A; Ruppel, Kathleen M; Bernstein, Daniel.
Afiliación
  • Vander Roest AS; Department of Pediatrics (Cardiology), Stanford University School of Medicine, Palo Alto, CA 94304.
  • Liu C; Department of Mechanical Engineering, Stanford University, Stanford, CA 94305.
  • Morck MM; Department of Bioengineering, School of Engineering and School of Medicine, Stanford University, Stanford, CA 94305.
  • Kooiker KB; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305.
  • Jung G; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305.
  • Song D; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305.
  • Dawood A; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305.
  • Jhingran A; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305.
  • Pardon G; Department of Pediatrics (Cardiology), Stanford University School of Medicine, Palo Alto, CA 94304.
  • Ranjbarvaziri S; School of Medicine, University of Washington, Seattle, WA 98109.
  • Fajardo G; Department of Pediatrics (Cardiology), Stanford University School of Medicine, Palo Alto, CA 94304.
  • Zhao M; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305.
  • Campbell KS; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305.
  • Pruitt BL; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305.
  • Spudich JA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305.
  • Ruppel KM; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305.
  • Bernstein D; Department of Pediatrics (Cardiology), Stanford University School of Medicine, Palo Alto, CA 94304.
Proc Natl Acad Sci U S A ; 118(24)2021 06 15.
Article en En | MEDLINE | ID: mdl-34117120
Hypertrophic cardiomyopathy (HCM) is the most common inherited form of heart disease, associated with over 1,000 mutations, many in ß-cardiac myosin (MYH7). Molecular studies of myosin with different HCM mutations have revealed a diversity of effects on ATPase and load-sensitive rate of detachment from actin. It has been difficult to predict how such diverse molecular effects combine to influence forces at the cellular level and further influence cellular phenotypes. This study focused on the P710R mutation that dramatically decreased in vitro motility velocity and actin-activated ATPase, in contrast to other MYH7 mutations. Optical trap measurements of single myosin molecules revealed that this mutation reduced the step size of the myosin motor and the load sensitivity of the actin detachment rate. Conversely, this mutation destabilized the super relaxed state in longer, two-headed myosin constructs, freeing more heads to generate force. Micropatterned human induced pluripotent derived stem cell (hiPSC)-cardiomyocytes CRISPR-edited with the P710R mutation produced significantly increased force (measured by traction force microscopy) compared with isogenic control cells. The P710R mutation also caused cardiomyocyte hypertrophy and cytoskeletal remodeling as measured by immunostaining and electron microscopy. Cellular hypertrophy was prevented in the P710R cells by inhibition of ERK or Akt. Finally, we used a computational model that integrated the measured molecular changes to predict the measured traction forces. These results confirm a key role for regulation of the super relaxed state in driving hypercontractility in HCM with the P710R mutation and demonstrate the value of a multiscale approach in revealing key mechanisms of disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cardiomiopatía Hipertrófica / Miosinas Ventriculares / Mutación / Contracción Miocárdica Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cardiomiopatía Hipertrófica / Miosinas Ventriculares / Mutación / Contracción Miocárdica Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos