Your browser doesn't support javascript.
loading
A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor.
Palomba, Emanuela; Tirelli, Valentina; de Alteriis, Elisabetta; Parascandola, Palma; Landi, Carmine; Mazzoleni, Stefano; Sanchez, Massimo.
Afiliación
  • Palomba E; Department of Research Infrastructures for marine biological resources (RIMAR), Stazione Zoologica "Anton Dohrn", Villa Comunale, Napoli, Italy.
  • Tirelli V; Istituto Superiore di Sanità (ISS) Core Facilities, Rome, Italy.
  • de Alteriis E; Department of Biology, University of Naples "Federico II", Naples, Italy.
  • Parascandola P; Department of Industrial Engineering, University of Salerno, Salerno, Italy.
  • Landi C; Department of Industrial Engineering, University of Salerno, Salerno, Italy.
  • Mazzoleni S; Department of Agricultural Sciences, University of Naples "Federico II", Naples, Italy.
  • Sanchez M; Istituto Superiore di Sanità (ISS) Core Facilities, Rome, Italy.
PLoS One ; 16(6): e0248382, 2021.
Article en En | MEDLINE | ID: mdl-34111115
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Reactores Biológicos / Técnicas de Cultivo Celular por Lotes / Fluorometría Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Reactores Biológicos / Técnicas de Cultivo Celular por Lotes / Fluorometría Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos