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Development and evaluation of a loop-mediated isothermal amplification assay for clinical diagnosis of respiratory human adenoviruses emergent in China.
Hong, Lei; Li, Jingyuan; Lv, Junping; Chao, Siqi; Xu, Yaqing; Zou, Dayang; Du, Junjie; Lu, Binan; Pang, Zongran; Li, Wenfeng; Liu, Wei; Ke, Yuehua; Hou, Shuxun.
Afiliación
  • Hong L; Department of Orthopedic Surgery, Chinese PLA General Hospital, Beijing, P. R. China; Department of Orthopedic Surgery, Fourth center of Chinese PLA General Hospital, Beijing, P. R. China.
  • Li J; Department of Orthopaedics, Air Force Clinical College(Air Force Medical Center) of Anhui Medical University, Beijing, P. R. China.
  • Lv J; Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing, P. R. China.
  • Chao S; Center for Disease Prevention and Control of PLA, Beijing, P. R. China.
  • Xu Y; Center for Disease Prevention and Control of PLA, Beijing, P. R. China.
  • Zou D; Center for Disease Prevention and Control of PLA, Beijing, P. R. China.
  • Du J; Department of Orthopaedics, Air Force Clinical College(Air Force Medical Center) of Anhui Medical University, Beijing, P. R. China.
  • Lu B; Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing, P. R. China.
  • Pang Z; Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing, P. R. China.
  • Li W; Department of Orthopedic Surgery, Chinese PLA General Hospital, Beijing, P. R. China; Department of Orthopedic Surgery, Fourth center of Chinese PLA General Hospital, Beijing, P. R. China.
  • Liu W; Center for Disease Prevention and Control of PLA, Beijing, P. R. China. Electronic address: 1212815@qq.com.
  • Ke Y; Center for Disease Prevention and Control of PLA, Beijing, P. R. China. Electronic address: yuehuakebj@163.com.
  • Hou S; Department of Orthopedic Surgery, Chinese PLA General Hospital, Beijing, P. R. China; Department of Orthopedic Surgery, Fourth center of Chinese PLA General Hospital, Beijing, P. R. China. Electronic address: tiantaindede@163.com.
Diagn Microbiol Infect Dis ; 101(1): 115401, 2021 Sep.
Article en En | MEDLINE | ID: mdl-34087512
Three human adenovirus (HAdV) genotypes, HAdV-7, HAdV-14, and HAdV-55, emerged as the most prevalent variants in China over the past decade and caused both sporadic, fatal cases and frequent, large outbreaks. Early diagnosis is essential to control infections and endemics. Here, we established a loop-mediated isothermal amplification (LAMP) assay coupled with an instrument-free nucleic acid extraction device recently developed by our group; the assay could detect all the 3 prevalent HAdV genotypes. Specificity analysis showed no cross-reactivity with other common respiratory pathogens and the analytical sensitivity was as low as 10 copies/µL. All detection steps could be completed within 1 hour. The assay's performance was evaluated using clinical samples and compared with the gold standard RT-PCR method, showing highly consistent results. The LAMP assay developed here could be readily used in basic laboratory facilities and with minimal DNA extraction equipment, and as a reliable screening test in a resource-limited setting.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones del Sistema Respiratorio / Infecciones por Adenovirus Humanos / Adenovirus Humanos / Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones del Sistema Respiratorio / Infecciones por Adenovirus Humanos / Adenovirus Humanos / Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos