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Healing of Experimental Periodontal Defects Following Treatment with Fibroblast Growth Factor-2 and Deproteinized Bovine Bone Mineral.
Murakami, Tasuku; Matsugami, Daisuke; Yoshida, Wataru; Imamura, Kentaro; Bizenjima, Takahiro; Seshima, Fumi; Saito, Atsushi.
Afiliación
  • Murakami T; Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.
  • Matsugami D; Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.
  • Yoshida W; Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.
  • Imamura K; Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.
  • Bizenjima T; Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.
  • Seshima F; Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan.
  • Saito A; Chiba Dental Center, Tokyo Dental College, Mihama-ku, Chiba 2618502, Japan.
Biomolecules ; 11(6)2021 05 29.
Article en En | MEDLINE | ID: mdl-34072351
The aim of this study was to investigate the effects of fibroblast growth factor (FGF)-2 used in combination with deproteinized bovine bone mineral (DBBM) on the healing of experimental periodontal defects. Periodontal defects created in rats were treated by FGF-2, DBBM, FGF-2 + DBBM, or left unfilled. Microcomputed tomography, histological, and immunohistochemical examinations were used to evaluate healing. In vitro cell viability/proliferation on DBBM with/without FGF-2 was assessed by WST-1. Cell behavior was analyzed using scanning electron and confocal laser scanning microscopy. Osteogenic differentiation was evaluated by staining with alkaline phosphatase and alizarin red. Bone volume fraction was significantly greater in FGF-2 and FGF-2 + DBBM groups than in other groups at 2 and 4 weeks postoperatively. In histological assessment, newly formed bone in FGF-2 and FGF-2 + DBBM groups appeared to be greater than other groups. Significantly greater levels of proliferating cell nuclear antigen-, vascular endothelial growth factor-, and osterix-positive cells were observed in FGF-2 and FGF-2 + DBBM groups compared to Unfilled group. In vitro, addition of FGF-2 to DBBM promoted cell viability/proliferation, attachment/spreading, and osteogenic differentiation. The combination therapy using FGF-2 and DBBM was similarly effective as FGF-2 alone in the healing of experimental periodontal defects. In certain bone defect configurations, the combined use of FGF-2 and DBBM may enhance healing via promotion of cell proliferation, angiogenesis, and osteogenic differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Periodoncio / Factor 2 de Crecimiento de Fibroblastos / Sustitutos de Huesos Límite: Animals Idioma: En Revista: Biomolecules Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Periodoncio / Factor 2 de Crecimiento de Fibroblastos / Sustitutos de Huesos Límite: Animals Idioma: En Revista: Biomolecules Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza